文献类型： 外文期刊
作者： Ma, T. H. 1 ; Xiong, Q. H. 1 ; Yuan, B. 1 ; Jiang, H. 1 ; Gao, Y. 1 ; Xu, J. B. 1 ; Liu, S. Y. 1 ; Ding, Y. 2 ; Zhang, G. L. 3 ;
作者机构： 1.Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130023, Peoples R China
2.Jilin Univ, Lab Anim Ctr, Changchun 130023, Peoples R China
3.Jilin Acad Agr Sci, Changchun, Peoples R China
关键词： Bovine;Leydig cells;LHR;Splicing variants
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2012 年 11 卷 2 期
页码：
收录情况： SCI
摘要： The luteinizing hormone receptor (LHR) plays a key role in testosterone production through its interaction with the gonadotropins, LH and chorionic gonadotropin. We examined the LHR splicing pattern in bovine Leydig cells; LH-induced expression of eight cloned splicing variants was detected by real-time PCR. Luteinizing hormone applied to cultured Leydig cells resulted in expression of full-length LHR and the A and B isoforms, as well as secretion of testosterone, which first increased, then declined, and then increased further, with increased LH levels. The secretion of testosterone progressively increased with increasing LH, but the expression levels of LHR (FL, A, and B) did not increase correspondingly. We conclude that the LHR splicing pattern is complex in bovine Leydig cells, and that expression of full-length LHR and isoforms A and B changes when induced with LH.
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