A screening method for detecting simple sequence repeat (SSR) polymorphism of Zea mays using high-resolution melting-curve analysis
文献类型： 外文期刊
作者： Yu, R. H. 2 ; Shan, X. H. 3 ; Wang, S. 2 ; Li, X. H. 2 ; Jiang, Y. 1 ; Tan, H. 1 ; Li, Y. D. 1 ;
作者机构： 1.Jilin Acad Agr Sci, Biotechnol Res Ctr, Changchun 130033, Peoples R China
2.Jilin Agr Univ, Coll Agron, Changchun 130118, Peoples R China
3.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China
关键词： High-resolution melting-curve;SSR;Zea mays
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2011 年 10 卷 73 期
页码：
收录情况： SCI
摘要： As a very important molecular marker technique, simple sequence repeat (SSR) is widely used in marker assistant selection and maize DNA fingerprint. High-resolution melting-curve (HRM) analysis is a new technique to detect the sequence differences in polymerase chain reaction (PCR) amplicons, which is quicker, more accurate and effective than electrophoresis analysis. In this study, we developed a HRM system to detect SSR polymorphism of Zea mays. We successfully used this method to identify genotypes of F2 generation and detecting the SSR polymorphism in different maize inbred lines, therefore, this HRM-SSR system can be used to substitute the electrophoresis after SSR PCR amplification.
- 相关文献
作者其他论文 更多>>
-
DESIGN AND APPLIED RESEARCH OF INDOOR SOILLESS PLANTING DEVICE FOR SPROUT-SEEDLING VEGETABLES IN FAMILY ROOMS
作者：Wang, S.;Zhang, X.;Feng, T.;Li, H.;Zhang, S.;Zhang, Z.;Wang, Y.;Ma, Y.;Feng, C.
关键词：Design; Family rooms; Indoor; Planting device; Soiless; Sprout-seedling vegetables
-
Meta-analysis of constitutive QTLs for disease resistance in maize and its synteny conservation in the rice genome
作者：Zhao, L.;Wang, Q. Y.;Liu, H. J.;Zhang, C. X.;Li, X. H.
关键词：Maize;QTL for disease resistance;Meta-analysis;Integrated linkage map;Comparative mapping;Genetic map
-
Thermodynamics of Cu2+ Adsorption on soil Humin
作者：Ji, F.;Zhang, J. J.;Wang, S.;Wang, L. C.
关键词：Humin;Humic acid;Cu2+;Adsorption isotherm;Thermodynamic parameters
-
Comparative analyses of genetic/epigenetic diversities and structures in a wild barley species (Hordeum brevisubulatum) using MSAP, SSAP and AFLP
作者：Liu, X. M.;Liu, B.;Li, Y. D.;Guo, W. L.;Shan, X. H.;Wu, Y.;Zhang, M. Z.;Yuan, Y. P.
关键词：Hordeum brevisubulatum;(Epi-)genetic diversity;(Epi-)genetic structure;Gene flow
-
Detection of differentially expressed genes in the longissimus dorsi of Northeastern Indigenous and Large White pigs
作者：Gao, Y.;Jiang, H.;Wang, S.;Sun, G. J.;Li, F. J.;Deng, Q.;Dai, L. S.;Cui, X. S.;Liu, D. F.;Zhang, J. B.;Zhang, Y. H.;Ma, Q.;Zhao, Z. H.;Xiao, S. Q.;Zhang, S. M.
关键词：Adipocyte fatty acid-binding protein;Gene expression;Longissimus dorsi;Meat quality;Pigs;Polymorphism
-
Fate of applied urea N-15 in a soil-maize system as affected by urease inhibitor and nitrification inhibitor
作者：Zhang, L.;Wu, Z.;Jiang, Y.;Chen, L.;Song, Y.;Zhang, L.;Wang, L.;Xie, J.;Ma, X.
关键词：nitrogen;fertilizer;N losses;N use efficiency
-
Variation in available micronutrients in black soil after 30-year fertilization treatment
作者：Zhang, Y. G.;Zhang, Y. Y.;Cai, J. P.;Jiang, Y.;Zhang, Y. G.;Zhu, P.;Gao, H. J.
关键词：
