文献类型： 外文期刊

作者： Yu Ying ¹ ; Liu Xiang-guo ² ; Lu Yang ¹ ; Dou Yao ¹ ; Wang Hu-yi ¹ ; Han Si-ping ² ; Feng Shu-dan ³ ; Hao Dong-yun ¹ ;

作者机构： 1.Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China

2.Jilin Acad Agr Sci, Biotechnol Res Ctr, Changchun 130033, Peoples R China

3.Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150080, Peoples R China

关键词： Chinese wildrye;Monocot;Salt stress;Type II peroxiredoxin

期刊名称：CHEMICAL RESEARCH IN CHINESE UNIVERSITIES （ 影响因子：1.307； 五年影响因子：0.979 ）

ISSN： 1005-9040

年卷期： 2011 年 27 卷 6 期

页码：

收录情况： SCI

摘要： Peroxiredoxins(Prxs) are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species(ROS). Although extensive efforts have been made over the last decades in understanding the structures and functions of Prxs, type II Prxs in monocots are hardly studied. In this work, a monocot type II Prx gene homologue from Chinese wildrye(Leymus Chinensis), designated as LcTpxII, was isolated and characterized. LcTpxII encoding a 162-amino acid protein contains a thioredoxin domain and a cysteine residue at position 51, suggesting that it is a member of the Prxs family. The LcTpxII is capable of decomposing H(2)O(2) and protecting plasmid DNA from damage caused by ROS. The expression of LcTpxII in Chinese wildrye was induced by 400 mmol/L NaCl and 100 mmol/L Na(2)CO(3) in the experiment. The overexpression of LcTpxII enhances the tolerance of transgenic yeast to 1.6 mol/L NaCl and 10 mmol/L Na(2)CO(3).