您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice

文献类型： 外文期刊

作者： Li, Yuan 1 ; Xia, Qiong 1 ; Kou, Hongping 3 ; Wang, Dan 3 ; Lin, Xiuyun 4 ; Wu, Ying 1 ; Xu, Chunming 1 ; Xing, Shaochen; 1 ;

作者机构： 1.NE Normal Univ, Key Lab Mol Epigenet MOE, Changchun 130024, Peoples R China

2.NE Normal Univ, Inst Genet & Cytol, Changchun 130024, Peoples R China

3.Jilin Agr Univ, Dept Agron, Changchun 130118, Peoples R China

4.Jilin Acad Agr Sci, Ctr Agribiotechnol, Changchun 130033, Peoples R China

关键词： DNA methylation;Magnaporthe grisea;blast-resistance;induced Pib expression

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2011 年 53 卷 10 期

页码：

收录情况： SCI

摘要： Pib is a well-characterized rice blast-resistance gene belonging to the nucleotide binding site (NBS) and leucine-rich repeat (LRR) superfamily. Expression of Pib was low under non-challenged conditions, but strongly induced by the blast-causing fungal pathogen Magnaporthe grisea, thereby conferring resistance to the pathogen. It is generally established that cytosine methylation of the promoter-region often plays a repressive role in modulating expression of the gene in question. We report here that two critical regions of the Pib promoter were heavily CG cytosine-methylated in both cultivars studied. Surprisingly, induced expression of Pib by M. grisea infection did not entail its promoter demethylation, and partial demethylation by 5-azacytidine-treatment actually reduced Pib expression relative to wildtype plants. Accordingly, the blast disease-resistance was compromised in the 5'-azaC-treated plants relative to wild-type. In contrast, the disease susceptibility was not affected by the 5'-azaC treatment in another two rice cultivars that did not contain the Pib gene, ruling out effects of other R genes and non-specific genotoxic effects by the drug-treatment as a cause for the compromised Pib-conditioned blast-resistance. Taken together, our results suggest that promoter DNA methylation plays a novel enhancing role in conditioning high-level of induced expression of the Pib gene in times of M. grisea infection, and its conferred resistance to the pathogen.

  • 相关文献

[1]Laser Irradiation-Induced DNA Methylation Changes Are Heritable and Accompanied with Transpositional Activation of mPing in Rice. Li, Siyuan,Xia, Qiong,Yu, Xiaoming,Gao, Xiang,Liu, Bao,Li, Siyuan,Wang, Fang,Ma, Jian,Kou, Hongping,Lin, Xiuyun. 2017

[2]Mutation of the RDR1 gene caused genome-wide changes in gene expression, regional variation in small RNA clusters and localized alteration in DNA methylation in rice. Wang, Ningning,Zhang, Di,Wang, Zhenhui,Xun, Hongwei,Wang, Hui,Huang, Wei,Liu, Ying,Li, Ning,Ou, Xiufang,Zhang, Chunyu,Liu, Bao,Wang, Ningning,Ma, Jian,Lin, Xiuyun,Zhang, Chunyu,Wang, Ningning,Wang, Ming-Bo. 2014

[3]Endosperm-specific hypomethylation, and meiotic inheritance and variation of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) inter-strain hybrids. Zhang, M. S.,Yan, H. Y.,Zhao, N.,Lin, X. Y.,Pang, J. S.,Xu, K. Z.,Liu, L. X.,Liu, B..

[4]Alien DNA Introgression into Rice Causes Heritable Alterations in DNA Methylation Patterns in an Active Retrotransposon Tos17. Dong, YZ,Liu, ZL,Dong, YS,Han, FR,He, MY,Hao, S,Liu, B.

[5]Comparative analysis on genome-wide DNA methylation in longissimus dorsi muscle between Small Tailed Han and DorperxSmall Tailed Han crossbred sheep. Cao, Yang,Zhao, Zhi-Hui,Cao, Yang,Jin, Hai-Guo,Ma, Hui-Hai.

[6]DNA Methylation Changes Induced in Rice by Exposure to High Concentrations of the Nitric Oxide Modulator, Sodium Nitroprusside. Ou, Xiufang,Zhuang, Tingting,Yin, Wenchao,Miao, Yiling,Wang, Bo,Zhang, Yunhong,Lin, Xiuyun,Xu, Chunming,Liu, Bao,Zhuang, Tingting,Lin, Xiuyun,von Wettstein, Diter,Rustgi, Sachin,von Wettstein, Diter,Rustgi, Sachin.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台