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Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice

文献类型： 外文期刊

作者： Li, Yuan 1 ; Xia, Qiong 1 ; Kou, Hongping 3 ; Wang, Dan 3 ; Lin, Xiuyun 4 ; Wu, Ying 1 ; Xu, Chunming 1 ; Xing, Shaochen; 1 ;

作者机构： 1.NE Normal Univ, Key Lab Mol Epigenet MOE, Changchun 130024, Peoples R China

2.NE Normal Univ, Inst Genet & Cytol, Changchun 130024, Peoples R China

3.Jilin Agr Univ, Dept Agron, Changchun 130118, Peoples R China

4.Jilin Acad Agr Sci, Ctr Agribiotechnol, Changchun 130033, Peoples R China

关键词： DNA methylation;Magnaporthe grisea;blast-resistance;induced Pib expression

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2011 年 53 卷 10 期

页码：

收录情况： SCI

摘要： Pib is a well-characterized rice blast-resistance gene belonging to the nucleotide binding site (NBS) and leucine-rich repeat (LRR) superfamily. Expression of Pib was low under non-challenged conditions, but strongly induced by the blast-causing fungal pathogen Magnaporthe grisea, thereby conferring resistance to the pathogen. It is generally established that cytosine methylation of the promoter-region often plays a repressive role in modulating expression of the gene in question. We report here that two critical regions of the Pib promoter were heavily CG cytosine-methylated in both cultivars studied. Surprisingly, induced expression of Pib by M. grisea infection did not entail its promoter demethylation, and partial demethylation by 5-azacytidine-treatment actually reduced Pib expression relative to wildtype plants. Accordingly, the blast disease-resistance was compromised in the 5'-azaC-treated plants relative to wild-type. In contrast, the disease susceptibility was not affected by the 5'-azaC treatment in another two rice cultivars that did not contain the Pib gene, ruling out effects of other R genes and non-specific genotoxic effects by the drug-treatment as a cause for the compromised Pib-conditioned blast-resistance. Taken together, our results suggest that promoter DNA methylation plays a novel enhancing role in conditioning high-level of induced expression of the Pib gene in times of M. grisea infection, and its conferred resistance to the pathogen.

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