文献类型： 外文期刊

作者： Kou, H. P. ² ; Li, Y. ² ; Song, X. X. ² ; Ou, X. F. ² ; Xing, S. C. ⁵ ; Ma, J. ⁴ ; Von Wettstein, D. ¹ ; Liu, B. ² ;

作者机构： 1.Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA

2.NE Normal Univ, Key Lab Mol Epigenet MOE, Changchun 130024, Peoples R China

3.NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Peoples R China

4.Jilin Agr Univ, Dept Agron, Changchun 130118, Peoples R China

5.Jilin Acad Agr Sci, Agri Biotechnol Ctr, Changchun 130124, Peoples R China

关键词： Abiotic stress;Adaptation;Cytosine methylation alteration;Epigenetic inheritance;Lamarckism;Nitrogen (N)-deficiency;Oryza sativa L.

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）

ISSN： 0176-1617

年卷期： 2011 年 168 卷 14 期

页码：

收录情况： SCI

摘要： Cytosine methylation is responsive to various biotic- and abiotic-stresses, which may produce heritable epialleles. Nitrogen (N)-deficiency is an abiotic stress being repeatedly experienced by plants. To address possible epigenetic consequences of N-deficiency-stress, we investigated the stability of cytosine methylation in rice (Oryza sativa L) subsequent to a chronic (a whole-generation) N-deficiency at two levels, moderate (20 mg/L) and severe (10 mg/L), under hydroponic culture. MSAP analysis revealed that locus-specific methylation alteration occurred in leaf-tissue of the stressed plants (So) experiencing either level of N-deficiency, which was validated by gel-blotting. Analysis on three non-stressed self-fed progenies (S(1), S(2) and S(3)) by gel-blotting indicated that ca. 50% of the altered methylation patterns in somatic cells (leaf) of the stressed S(0) plants were recaptured in S(1), which were then stably inherited to S(2) and S(3). Bisulfite sequencing of two variant MSAP loci with homology to low-copy retrotransposons on one stressed plant (So) and its non-stressed progenies (S(1) and S(2)) showed that whereas one locus exhibited limited and non-heritable CHH methylation alteration, the other locus manifested dramatic heritable hypermethylation at nearly all cytosine sites within the assayed region. Intriguingly, when two groups of S(2) plants descended from the same N-deficiency-stressed So plant were re-subjected to the stress, the group inheriting the modified methylation patterns showed enhanced tolerance to the Ndeficiency-stress compared with the group bearing the original patterns. Our results thus demonstrate heritability of an acquired adaptive trait in rice, which was accompanied by epigenetic inheritance of modified cytosine methylation patterns, implicating an epigenetic basis underlying the inheritance of an acquired trait in plants. (C) 2011 Elsevier GmbH. All rights reserved.