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Heritable alteration in DNA methylation pattern occurred specifically at mobile elements in rice plants following hydrostatic pressurization

文献类型: 外文期刊

作者: Long, LK 1 ; Lin, XY 2 ; Zhai, JZ 2 ; Kou, HP 2 ; Yang, W 2 ; Liu, B 3 ;

作者机构: 1.NE Normal Univ, Lab Plant Mol Epigenet, Inst Genet & Cytol, Changchun 130024, Peoples R China

2.NE Normal Univ, Lab Plant Mol Epigenet, Inst Genet & Cytol, Changchun 130024, Peoples R China; Jilin Acad Agr Sci, Gongzuling 136100, Peoples R China; Jilin Agr Univ, Dept Agron, Changchun 130118, Peoples R China

3.NE Normal Univ, Lab Plant Mol Epigenet, Inst Genet & Cytol, Changchun 130

关键词: hydrostatic pressured;mobile elements;DNA methylation alteration;epigenetic inheritance;rice

期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )

ISSN: 0006-291X

年卷期: 2006 年 340 卷 2 期

页码:

收录情况: SCI

摘要: Intrinsic DNA methylation pattern is an integral component of the epigenetic network in many eukaryotes. Exploring the extent to which DNA methylation patterns can be altered under a specific condition is important for elucidating the biological functions of this epigenetic modification. This is of added significance in plants wherein the newly acquired methylation patterns can be inherited through organismal generations, We report here that DNA methylation patterns of mobile elements but not of cellular genes were specifically altered in rice plants following hydrostatic pressurization. This was evidenced by methylation-sensitive gel-blot analysis, which showed that 10 out of 10 studied low-copy transposons and retrotransposons manifested methylation alteration in at least one of the 8 randomly chosen pressure-treated plants, whereas none of the 16 studied low-copy cellular genes showed any change. Both gel-blotting and genome-wide fingerprinting indicated that the methylation alteration in mobile elements was not accompanied by a general genetic instability. Progeny analysis indicated retention of the altered methylation patterns in most progeny plants, underscoring early occurrence of the alterations, and their faithful epigenetic inheritance. (c) 2005 Elsevier Inc. All rights reserved.

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