文献类型： 外文期刊

作者： Zhao, GL ¹ ; Liu, YZ ¹ ; Yin, AP ¹ ; Li, JB ¹ ;

作者机构： 1.Jilin Acad Agr Sci, Prov Key Open Lab Biotechnol, Gongzhuling 136100, Peoples R China

关键词： Glycine max L.;anther culture;embryo;germination

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 1998 年 43 卷 23 期

页码：

收录情况： SCI

摘要： A large number of experiments about induction of callus, embryo and germination of regeneration plants were done in soybean anther culture. Screening for genotypes, low-temperature pretreatment for floral buds, constituent of hormone in medium and effects of different temperatures on induction rate of callus and embryo were studied. It was found that the TDZ played an important role in embryo induction and germination. 28 genotypes had reaction competent to the anther culture in all 110 genotypes. Induction rate of callus was 26%-68.1%. Plant differentiation rate was 0.5%-2.0%.