文献类型： 外文期刊
作者： Wang, Fang 1 ; Sun, Yu 2 ; Ruan, Jishou 3 ; Chen, Rui 4 ; Chen, Xin 2 ; Chen, Chengjie 5 ; Kreuze, Jan F. 6 ; Fei, ZhangJ 1 ;
作者机构： 1.Tianjin Med Univ, Hosp 2, Dept Gynaecol, Tianjin 300211, Peoples R China
2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
3.Nankai Univ, Sch Math Sci, Tianjin 300071, Peoples R China
4.Tianjin Acad Agr Sci, Tianjin Inst Agr Qual Stand & Testing Technol, Tianjin 300381, Peoples R China
5.South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
6.CIP, Apartado 1558, Lima 12, Peru
7.Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA
8.Guangdong Med Univ, Dongguan Sci Res Ctr, Guangdong Prov Key Lab Med Mol Diagnost, Dongguan 523808, Guangdong, Peoples R China
期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）
ISSN： 2314-6133
年卷期： 2016 年
页码：
收录情况： SCI
摘要： Small RNA sequencing (sRNA-seq) can be used to detect viruses in infected hosts without the necessity to have any prior knowledge or specialized sample preparation. The sRNA-seq method was initially used for viral detection and identification in plants and then in invertebrates and fungi. However, it is still controversial to use sRNA-seq in the detection of mammalian or human viruses. In this study, we used 931 sRNA-seq runs of data from the NCBI SRA database to detect and identify viruses in human cells or tissues, particularly from some clinical samples. Six viruses including HPV-18, HBV, HCV, HIV-1, SMRV, and EBV were detected from 36 runs of data. Four viruses were consistent with the annotations from the previous studies. HIV-1 was found in clinical samples without the HIV-positive reports, and SMRV was found in Diffuse Large B-Cell Lymphoma cells for the first time. In conclusion, these results suggest the sRNA-seq can be used to detect viruses in mammals and humans.
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