Establishment and Characteristics of the Spermatogonial Stem Cell Line from the Yellow River Carp (Cyprinus carpio haematopterus)
文献类型： 外文期刊
作者： Zhou, Huijie 1 ; Liu, Tianqi 1 ; Zhang, Tan 1 ; Sun, Zhipeng 1 ; Xu, Huan 1 ; Zhang, Tingting 1 ; Yin, Yashan 1 ; Li, Na 1 ; Yan, Ting 1 ; Kuang, Youyi 1 ;
作者机构： 1.Natl & Local Joint Engn Lab Freshwater Fish Breedi, 232,Hesong St, Harbin 150070, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Biotechnol & Breeding, 232,Hesong St, Harbin 150070, Peoples R China
3.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, 232,Hesong St, Harbin 150070, Peoples R China
4.Tianjin Agr Univ, Coll Fisheries, 22,Jinjing Rd, Tianjin 300384, Peoples R China
5.Dalian Ocean Univ, Coll Fisheries & Life Sci, 52,Heishijiao St, Dalian 116023, Peoples R China
关键词：
期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN：
年卷期： 2025 年 14 卷 5 期
页码：
收录情况： SCI
摘要： To address the growing consumer demands for improved fish meat quality, desirable morphological traits, and sustainable production practices, researchers have intensified efforts in the selective breeding and genetic improvement of carp (Cyprinus carpio) varieties. However, traditional breeding methods are often time-consuming and inefficient, which poses challenges to the sustainable development of the carp aquaculture industry. The establishment of germ stem cell lines offers a crucial tool for the study of germ cells, genetic improvement, and species conservation. In this study, we successfully established a spermatogonial stem cell line (YRSSCs) from Yellow River carp (Cyprinus carpio haematopterus) that can be cultured in vitro for the long term. We optimized the culture conditions to maintain their self-renewal and differentiation capabilities. The results demonstrated that YRSSCs have a diploid karyotype and can stably proliferate for over a year in L-15 medium supplemented with 5 mmol/L HEPES, 50 mu mol/L beta-mercaptoethanol, 15% FBS, 2 ng/mL bFGF, 2 ng/mL LIF, 1% carp serum, 800 IU/mL penicillin, 0.8 mg/mL streptomycin, 2 mu g/mL amphotericin B, 1% zebrafish embryo extract, and 1% glutamine at 30 degrees C in the absence of CO2. The cells exhibited a typical germ stem cell gene expression profile, with strong expression of the vasa, plzf-a, and Oct4-a genes. Additionally, this study found that YRSSCs possess the ability to differentiate in vitro and functionally colonize in vivo within recipient bodies. This research explored the establishment of YRSSCs and their differentiation potential both in vitro and in vivo, providing a novel strategy for the genetic improvement of aquaculture fish species through germ stem cell-based gene editing and transplantation technologies.
- 相关文献
作者其他论文 更多>>
-
Comprehensive Evaluation of Growth Performance, Hematological Parameters, Antioxidant Capacity, Innate Immunity, and Disease Resistance in Crucian Carp (Carassius auratus) Lacking Intermuscular Bones
作者：Zhao, Ran;Zhu, Jianqiang;Li, Shaowu;Sun, Zhipeng;Zhang, Tingting;Wang, Jing;Zheng, Xianhu;Kuang, Youyi;Wang, Di
关键词：Carassius auratus; a new strain lacking intermuscular bones; growth performance; iron overload; immune function
-
Transcriptome analysis reveals the candidate genes associated with skin color variation in Luciobarbus capito
作者：Geng, Longwu;Shang, Xinchi;Wei, Haijun;Tong, Guangxiang;Xu, Wei;Kuang, Youyi;Geng, Longwu;Shang, Xinchi;Wei, Haijun;Tong, Guangxiang;Xu, Wei;Kuang, Youyi
关键词：Transcriptomics; Pigmentation; Melanin; Carotenoids; Luciobarbus capito
-
Inclusion of macroalgae in the diet - a comparative survey from Norway, Chile and China
作者：Goecke, Franz;Ergon, Ashild;Aakre, Inger;Angarita, Lisse;Escobar, Maria Cristina;Cisternas, Silvana;Li, Na;Wang, Lianzhu;Li, Xiaodong;Pang, Shaojun
关键词：seaweed; nutrition; seafood; iodine; food risks; culinary traditions
-
Chromosome-level draft genome assembly of Hypomesus nipponensis reveals transposable element expansion reshaping the genome structure
作者：Zhu, Chenzhao;Tang, Fujiang;Zhu, Chenzhao;Kuang, Youyi;Li, Zhe;Tang, Fujiang
关键词：
Hypomesus nipponensis ; genome; evolutionary; gene family; repeat sequence -
Multiple comparative analysis revealed genetic basis of residual feed intake trait in common carp (Cyprinus carpio L.)
作者：Xu, Yihua;Sun, Zhipeng;Liu, Tianqi;Na, Rongbin;Zhang, Kexin;Zheng, Xianhu;Lu, Cuiyun;Xu, Yihua;Zheng, Xianhu;Lu, Cuiyun
关键词：Cyprinus carpio L.; Residual feed intake; Histological analysis; Digestive enzyme activity; Comparative transcriptome
-
Cottonseed protein concentrate as an effective substitute to fish meal in pike perch (Sander luciperca) feed: evidence from growth performance and intestinal responses of immune function and microflora
作者：Fan, Ze;Zhao, Jie;Lu, Cuiyun;Wu, Di;Sun, Zhipeng;Li, Jinnan;Wang, Liansheng;Zheng, Xianhu;Zhao, Jie;Huang, Jiaming
关键词：pikeperch (
Sander luciperca ); cottonseed protein concentrate (CPC); 60% substitution ratio for fish meal; decreasedigf1 expression; intestinal microflora -
Dynamic Impacts of Stock Enhancement on Kaluga Sturgeon (Huso dauricus): Novel Conservation Strategy Insights from the Gut Microbe Composition and Gene Expression Mode
作者：Li, Yutao;Wang, Ruoyu;Cao, Dingchen;Zhang, Ying;Zhai, Cunhua;Sun, Zhipeng;Ma, Bo
关键词：stock enhancement; sturgeon; environmental adaptation; gut microbiota; conservation biology
