文献类型： 外文期刊

作者： Yu, Ying ¹ ; Tang, Xinming ⁵ ; Duan, Chunhui ¹ ; Suo, Jingxia ¹ ; Crouch, Colin ⁶ ; Zhang, Sixin ¹ ; Liu, Xianyong ¹ ; Liu, Jie ¹ ; Bruton, Beth ⁶ ; Tarpey, Ian ⁶ ; Suo, Xun ¹ ;

作者机构： 1.Natl Key Lab Vet Publ Hlth & Safety, Beijing, Peoples R China

2.Minist Agr, Key Lab Anim Epidemiol & Zoonosis, Beijing, Peoples R China

3.China Agr Univ, Natl Anim Protozoa Lab, Beijing, Peoples R China

4.China Agr Univ, Coll Vet Med, Beijing, Peoples R China

5.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Key Lab Anim Biosafety Risk Prevent & Control Nort, Yinchuan, Ningxia, Peoples R China

6.MSD Anim Hlth, Milton Keynes, England

关键词： Eimeria acervulina; surface; microneme; immune responses; viral antigen

期刊名称：INFECTION AND IMMUNITY （ 影响因子：3.1； 五年影响因子：3.2 ）

ISSN： 0019-9567

年卷期： 2024 年 92 卷 2 期

页码：

收录情况： SCI

摘要： Using transgenic Eimeria spp. to deliver exogenous antigens is a viable option for developing multivalent live vaccines. Previous research revealed that the location of antigen expression in recombinant Eimeria dictates the magnitude and type of immune responses. In this study, we constructed genetically modified Eimeria acervulina that expressed VP2 protein, a protective antigen from infectious bursal disease virus (IBDV), on the surface or in the microneme of sporozoites. After vaccination, VP2-specific antibody was readily detected in specific pathogen-free chickens receiving transgenic E. acervulina parasites expressing VP2 in microneme, but animals vaccinated with which expressing VP2 on surface failed to produce detectable antibody after two times immunizations. Moreover, the bursal lesion of microneme-located VP2 transgenic E. acervulina immunized chickens was less severe compared with un-immunized animals after IBDV challenge infection. Therefore, genetically modified E. acervulina that express IBDV-derived VP2 in micronemes are effective in inducing specific antibody responses against VP2, while parasites that have VP2 expression on cell surface are not suitable. Thus, the use of Eimeria parasites as vaccine vectors needs to consider the proper targeting of exogenous immunogens. Our results have implications for the design of other vector vaccines.