文献类型： 外文期刊

作者： Li, Juan ¹ ; Wang, Dan-Dan ² ; Xu, Xiao-Shuang ⁴ ; Bai, Lu ² ; Peng, Bing ⁵ ; Pu, Ya-Jie ² ; Tian, Hong-Ling ⁶ ; Qin, Xue ¹ ;

作者机构： 1.Shanxi Pharmaceut Vocat Coll, Dept Pharm, Taiyuan 030031, Shanxi, Peoples R China

2.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Peoples R China

3.Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Peoples R China

4.Jiangsu Univ Technol, Sch Elect & Informat Engn, Inst Bioinformat & Med Engn, Changzhou 213001, Peoples R China

5.Capital Med Univ, Beijing Hosp Tradit Chinese Med, Beijing Inst Tradit Chinese Med, Beijing 100010, Peoples R China

6.Shanxi Acad Agr Sci, Res Inst Econ Crop, Fenyang 032200, Peoples R China

7.Shanxi Univ Tradit Chinese Med, Taiyuan 030024, Shanxi, Peoples R China

关键词： Polygala tenuifolia;Agronomic traits;UPLC/Q-TOF-MS;AFLP;Metabolomics

期刊名称：CHEMISTRY & BIODIVERSITY （ 影响因子：2.408； 五年影响因子：2.351 ）

ISSN： 1612-1872

年卷期： 2017 年 14 卷 9 期

页码：

收录情况： SCI

摘要： As one of the most important traditional Chinese medicine, the quality of Polygala tenuifolia is difficult to control and a new method must be established to facilitate/assist the breeding of P.tenuifolia. In this study, UPLC/Q-TOF-MS-based metabolomics analysis was performed to determine the chemical composition and screen metabolite biomarkers according to agronomic traits. A total of 29 compounds and 18 metabolite biomarkers were found. AFLP-based marker-assisted selection (MAS) was used to identify molecular marker bands and screen characteristic bands associated with specific agronomic traits. 184 bands and 76 characteristic AFLP bands were found. The correlation network between compounds and characteristic AFLP bands was built, so we may directly breed certain P.tenuifolia herbs with special agronomic traits (or characteristic AFLP bands), which exhibit specific pharmacological functions depending on the content of the active compounds. The proposed method of metabolomics coupled with MAS could facilitate/assist the breeding of P.tenuifolia.