Cloning and expression analysis of SPL8 homolog from pak choi (Brassica rapa subsp chinensis)
文献类型： 外文期刊
作者： Zhang, Jing 1 ; Ping, A-Min 1 ; Wang, Xue-Ting 1 ; Li, Gai-Zhen 1 ; Zhu, Zhu-Jun 3 ; Li, Mei-Lan 1 ; Xing, Guo-Ming 4 ; H 1 ;
作者机构： 1.Shanxi Agr Univ, Coll Hort, Dept Basic Sci, Taigu, Peoples R China
2.Shanxi Acad Agr Sci, Inst Vegetable Res, Taiyuan, Shanxi, Peoples R China
3.Zhejiang A&F Univ, Key Lab Qual Improvement Agr Prod Zhejiang Prov, Dept Hort, Coll Agr & Food Sci, Hangzhou, Zhejiang, Peoples R China
4.Shanxi Agr Univ, Coll Hort, Dept Vegetables, Taigu, Peoples R China
关键词： Brassica rapa subsp;chinensis;BrcSPL8;cloning;expression
期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）
ISSN： 1310-2818
年卷期： 2017 年 31 卷 6 期
页码：
收录情况： SCI
摘要： SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) transcription factor genes are functionally diverse; they control a number of fundamental aspects of plant growth and development, including vegetative phase change, flowering time, branching and leaf initiation rate. In our previous study, expression profiling showed that Bra033221, a transcript-derived fragment of an AtSPL8 ortholog, was up-regulated at flower bud differentiation stage 5. This result suggested that Bra033221 has a function similar to that of AtSPL8. In the present study, BrcSPL8, an AtSPL8 homolog, was cloned from pak choi (Brassica rapa subsp. chinensis) based on Bra033221 using reverse transcription-polymerase chain reaction (RT-PCR). The full-length cDNA was 1117 bp and contained a complete open reading frame (ORF) of 987 bp; this ORF encoded a predicted protein with 328 amino acid residues, a calculated molecular mass of 36.55 kDa and an isoelectric point of 8.85. BrcSPL8 was expressed in all analysed apices. Its expression levels before flower differentiation stage 1 were low and almost invariable, and the highest expression was detected in the apex at flower differentiation stage 5, suggesting that BrcSPL8 has a role during flower development in pak choi.
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