Expression analysis of two reverse duplicated small heat shock protein genes in rice (Oryza sativa L.)
文献类型： 外文期刊
作者： Guo Hong-xia 1 ; Zeng Wen-zhi 1 ; Wang Chuang-yun 3 ; Feng Jing-lei 1 ; Tang Hui-wu 1 ; Bai Mei 1 ; Liu Yao-guang 1 ; Zha 1 ;
作者机构： 1.South China Agr Univ, Coll Life Sci, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China
2.Shanxi Acad Agr Sci, Inst Crop Sci, Taiyuan 030031, Peoples R China
3.Shanxi Acad Agr S
关键词： small heat shock proteins (sHSPs);expression characteristics;semi-quantitative RT-PCR;GUS staining;rice
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2016 年 15 卷 8 期
页码：
收录情况： SCI
摘要： The small heat shock protein (sHSP) chaperones are required for protecting cellular proteins from damage, as well as refolding denatured proteins. This study was carried out to investigate the temporal-spatial expression patterns of two sHSP genes in rice. These two genes, named as 0s16.9A and 0s16.98, are reverse duplicated genes that adjacently located on chromosome 1 and probably share the same or overlapping DNA region as a promoter. The interval sequence between the start codons of the two genes which are transcribed in opposite directions is only about 2.6 kb. Semi-quantitative RT-PCR was carried out to detect the expression of the two genes under normal growth conditions and different stress conditions. The expression patterns of the two genes were in detail investigated by using beta-glucuronidase (GUS) reporter gene fusion system. Results showed that heat shock stress can induce high level expression of the two genes. Under normal growth conditions, 0s16.9A and 0s16.913 expressed in vegetative organs and young panicles. GUS staining combined with cytological observations showed that the two genes expressed mainly in the vascular tissues of roots, stems and young panicles, implicating that 0s16.9A and 0s16.98 play important roles not only for heat shock response, but also for normal development in rice.
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