A Study of Miriplatin and Its Pt-metabolites in Beagle Dog Plasma by Size Exclusion Chromatography-Inductively Coupled Plasma Mass Spectrometry
文献类型： 外文期刊
作者： Liu De-Ye 1 ; Hao Yuan-Bin 2 ; Han Wen-Ru 3 ; Li Jian 2 ; Huo Zong-Li 1 ; Liu Hua-Liang 1 ;
作者机构： 1.Jiangsu Prov Ctr Dis Control & Prevent, Nanjing 210009, Jiangsu, Peoples R China
2.Jiangsu Tripod Preclin Res Labs inc, Nanjing 210009, Jiangsu, Peoples R China
3.Shanxi Acad Agr Sci, Inst Anim Husb & Vet, Taiyuan 030032, Peoples R China
关键词： Miriplatin;Platinum-metabolites;Speciation;Pharmacokinetics
期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）
ISSN： 0253-3820
年卷期： 2014 年 42 卷 11 期
页码：
收录情况： SCI
摘要： A study of Pt-metabolites from a new anti. hepatoma drug miriplatin was important to miriplatin's pharmacology research. Therefore, a method based on size exclusion chromatography. inductively coupled plasma mass spectrometry (SEC-ICP-MS) was developed to study miriplatin and its Pt-metabolites in Beagle dog plasma. This method could be used to study total platinum concentration half. quantitatively. Compared with traditional ICP-MS direct determination, data acquired from this SEC-ICP-MS method were almost the same. By using BioSep-s2000 column, 25 mmol/L of pH 7.2 phosphate buffer as eluent, and Pt-195 as detecting isotope, we discovered miriplatin with its four Pt-metabolites in dog plasma after intra. hepatic artery administration. The main Pt-metabolite was m(2), which associated with plasma proteins. Miriplatin in plasma did not bind with plasma proteins. According to calculation, the ratio of miriplatin/m(2) first decreased rapidly, and then slowly increased to its second climax, finally slowly decreased.
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