文献类型： 外文期刊

作者： Qin, Huibin ¹ ; Lang, Huihua ¹ ; Yang, Hongjiang ¹ ;

作者机构： 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China

2.Shanxi Acad Agr Sci, Inst Crop Germplasm Resources, Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Taiyuan 030031, Peoples R China

3.Xingtai Inst Food & Drug Control, Xingtai 054001, Peoples R China

关键词： Methanogen community;16S rRNA gene library;mcrA gene library;Household anaerobic digester

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2013 年 97 卷 18 期

页码：

收录情况： SCI

摘要： Household anaerobic digesters have been installed across rural China for biogas production, but information on methanogen community structure in these small biogas units is sparsely available. By creating clone libraries for 16S rRNA and methyl coenzyme M reductase alpha subunit (mcrA) genes, we investigated the methanogenic consortia in a household biogas digester treating swine manure. Operational taxonomic units (OTUs) were defined by comparative sequence analysis, seven OTUs were identified in the 16S rRNA gene library, and ten OTUs were identified in the mcrA gene library. Both libraries were dominated by clones highly related to the type strain Methanocorpusculum labreanum Z, 64.0 % for 16S rRNA gene clones and 64.3 % for mcrA gene clones. Additionally, gas chromatography assays showed that formic acid was 84.54 % of the total volatile fatty acids and methane was 57.20 % of the biogas composition. Our results may help further isolation and characterization of methanogenic starter strains for industrial biogas production.