Transformation of trehalose synthase gene (TPS Gene) into corn inbred line and identification of drought tolerance
文献类型: 外文期刊
作者: Dong Chun-lin 1 ; Zhang Ming-yi 1 ; Zhang Yan-qin 1 ; Yang Li-li 1 ; Liang Gai-mei 1 ; Sun Jie 1 ; Lin Zhong-ping 2 ; Gou 1 ;
作者机构: 1.Shanxi Acad Agr Sci, Dryland Agr Res Ctr, Taiyuan 030006, Peoples R China
2.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
3.Shanxi Acad Agr Sci, Inst Informat, Taiyuan 030006, Peoples R China
关键词: Maize;trehalose synthase gene (TPS Gene);drought resistance
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 68 期
页码:
收录情况: SCI
摘要: Trehalose synthase gene (TPS gene) was transferred into maize inbred line Zheng58 with the improved pollen tube path method. At the three to five leaf stage, selection with spray Basta (0.7%) solution was conducted, and was then followed by polymerase chain reaction (PCR) and PCR-southern detection. The results showed that the new gene was transferred into the plants and integrated into the plant chromosomes. The initial drought-tolerance test in farmland confirmed that the drought resistance of transgenic plants was higher than that of non-transgenic plants. The results show that the new maize drought resistance germplasm can be gained by means of pollen tube path which is a feasible, effective and new way for maize drought resistance breeding.
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