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Effects of components in culture medium on glutamate decarboxylase activity and gamma-aminobutyric acid accumulation in foxtail millet (Setaria italica L.) during germination

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文献类型：外文期刊

作者： Bai, Qingyun ¹ ; Chai, Meiqing ³ ; Gu, Zhenxin ¹ ; Cao, Xiaohong ¹ ; Li, Yan ¹ ; Liu, Kunlun ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China

2.Huaiyin Inst Technol, Coll Life Sci & Chem Engn, Huaian 223001, Peoples R China

3.Shanxi Acad Agr Sci, Inst Special Fishery, Taiyuan 030031, Peoples R China

关键词： Foxtail millet;Germination;GABA;GAD;Culture medium;Optimisation

期刊名称：FOOD CHEMISTRY （影响因子：7.514；五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2009 年 116 卷 1 期

页码：

收录情况： SCI

摘要： The effects of glutamic acid (Glu), pyridoxal-5-phosphate (PLP) and calcium chloride (CaCl(2)) in culture medium on glutamate decarboxylase (GAD) activity and gamma-aminobutyric acid (GABA) accumulation in foxtail millet (Setaria italica L.) during germination were investigated in this study. The components in Culture medium for GABA accumulation were optimised using response Surface methodology (RSM). Results showed that GAD activity and GABA yield were dependent on the addition of Glu, PLP and CaCl(2) into the culture medium. Box-Behnken design indicated that the optimal culture components for GABA accumulation were: Glu at a concentration of 1.2 mg/ml, PLP at a concentration of 50 mu M and CaCl(2) at a concentration of 2.5 mM. Under the optimal conditions, the maximal production of GABA (42.9 mg/100 g FW) was obtained. Analysis of variance for the regression model suggested that the model can quite exactly predict GABA accumulation in millet during germination. (C) 2009 Elsevier Ltd. All rights reserved.

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