文献类型： 外文期刊
作者： Bai, Yunfeng 1 ; Guo, Zhihua 1 ; Wang, Xiaoqi 1 ; Bai, Dongmei 1 ; Zhang, Weifeng 1 ;
作者机构： 1.Shanxi Acad Agr Sci, Inst Crop Genet, Taiyuan 030031, Peoples R China
关键词： Solanum tuberosum;Marker-free;RNA silencing;Double-virus resistance;Transgene
期刊名称：PROGRESS IN NATURAL SCIENCE （ 影响因子：3.607； 五年影响因子：4.795 ）
ISSN： 1002-0071
年卷期： 2009 年 19 卷 5 期
页码：
收录情况： SCI
摘要： Viruses are very widespread in nature, and they cause severe diseases and yield losses in potato production. The transfer of the dsRNA-producing gene could confer a high level virus resistance by specific targeting of cognate viral RNA. In this study, we constructed a marker-free expression vector of a chimeric gene derived from the coat protein sequence of Potato virus X (PVX) and the nuclear inclusion protein sequence of Potato virus Y (PVY) in the form of an intramolecular dsRNA. Then this chimeric gene was introduced into potato cv. Zihuabai, a popular variety in China, via Agrobacterium tumefaciens-mediated transformation. Marker gene-free transgenic plants resistant to both PVX and PVY were obtained and confirmed by RT-PCR and DAS-ELISA detection. Northern blot analysis showed that transgene-derived mRNA was cleaved into short interfering RNAs (siRNAs), and that the virus resistance was mediated by RNA silencing. One important aspect of the study is that the transgenic viral sequence is not translated and the actual RNA transcript is cleaved, which possibly limit the environmental risks, such as transcapsidation and recombination of the transgene with an incoming virus. In addition, the biosafety risk resulting from marker genes can be avoided because of the absence of marker genes in transgenic plants. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Synergistic effect of imazapic plus prometryn on weeds in peanut (Arachis hypogaea L.) fields
作者：Du, Long;Li, Xiao;Jiang, Xiaojing;Ju, Qian;Qu, Chunjuan;Qu, Mingjing;Wang, Xiaoqiang;Bai, Dongmei;Wang, Jinxin
关键词：Imazapic; Prometryn; Combinatorial interaction; Weed control; Arachis hypogaea L
-
Pursuing sustainable productivity with millions of smallholder farmers
作者：Zhang, Hongyan;Chen, Xinping;Zhang, Chaochun;Huang, Chengdong;Zhang, Weifeng;Mi, Guohua;Miao, Yuxin;Li, Xiaolin;Ying, Hao;Yin, Yulong;Jiao, Xiaoqiang;Zhang, Qingsong;Fan, Mingsheng;Jiang, Rongfeng;Zhang, Fusuo;Ma, Wenqi;Gao, Qiang;Yang, Jianchang;Wang, Zhaohui;Tong, Yanan;Ye, Youliang;Guo, Shiwei;Lu, Jianwei;Huang, Jianliang;Lv, Shihua;Sun, Yixiang;Liu, Yuanying;Peng, Xianlong;Ren, Jun;Li, Shiqing;Deng, Xiping;Shi, Xiaojun;Zhang, Qiang;Yang, Zhiping;Tang, Li;Wei, Changzhou;Jia, Liangliang;Zhang, Jiwang;He, Mingrong;Tang, Qiyuan;Zhong, Xuhua;Liu, Zhaohui;Cao, Ning;Kou, Changlin;Dou, Zhengxia
关键词：
-
Pursuing sustainable productivity with millions of smallholder farmers
作者：Zhang, Hongyan;Chen, Xinping;Zhang, Chaochun;Huang, Chengdong;Zhang, Weifeng;Mi, Guohua;Miao, Yuxin;Li, Xiaolin;Ying, Hao;Yin, Yulong;Jiao, Xiaoqiang;Zhang, Qingsong;Fan, Mingsheng;Jiang, Rongfeng;Zhang, Fusuo;Ma, Wenqi;Gao, Qiang;Yang, Jianchang;Wang, Zhaohui;Tong, Yanan;Ye, Youliang;Guo, Shiwei;Lu, Jianwei;Huang, Jianliang;Lv, Shihua;Sun, Yixiang;Liu, Yuanying;Peng, Xianlong;Ren, Jun;Li, Shiqing;Deng, Xiping;Shi, Xiaojun;Zhang, Qiang;Yang, Zhiping;Tang, Li;Wei, Changzhou;Jia, Liangliang;Zhang, Jiwang;He, Mingrong;Tang, Qiyuan;Zhong, Xuhua;Liu, Zhaohui;Cao, Ning;Kou, Changlin;Dou, Zhengxia
关键词：
-
Producing more grain with lower environmental costs
作者：Chen, Xinping;Cui, Zhenling;Fan, Mingsheng;Wang, Guiliang;Wu, Liang;An, Ning;Wu, Liangquan;Ma, Lin;Zhang, Weifeng;Zhang, Fusuo;Vitousek, Peter;Zhao, Ming;Zhang, Weijian;Ma, Wenqi;Wang, Zhenlin;Zhang, Jiwang;He, Mingrong;Yan, Xiaoyuan;Yang, Jianchang;Deng, Xiping;Li, Shiqing;Gao, Qiang;Zhang, Qiang;Guo, Shiwei;Ren, Jun;Ye, Youliang;Zhu, Yunji;Wang, Zhaohui;Xue, Jiquan;Huang, Jianliang;Tang, Qiyuan;Sun, Yixiang;Peng, Xianlong
关键词：
