Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp trichophorum
文献类型: 外文期刊
作者: Yang, ZJ 1 ; Li, GR 2 ; Chang, ZJ 2 ; Zhou, JP 2 ; Ren, ZL 3 ;
作者机构: 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Sichuan, Peoples R China
2.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Sichuan, Peoples R China; Sichuan Agr Univ, Triticeae Res Inst, Dujiangyan 611830, Sichuan, Peoples R China; Shanxi Acad Agr Sci, Crop Genet Inst, Shanxi 030031, Peoples R China
3.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Sichuan, Peoples R China; Sichuan Agr Univ, Triticeae Res Inst, Dujiangyan 611830, Sichuan, Peoples R
关键词: C-banding;genomic in situ hybridization;partial amphiploid;Thinopyrum intermedium ssp;trichophorum;wheat
期刊名称:EUPHYTICA ( 影响因子:1.895; 五年影响因子:2.181 )
ISSN: 0014-2336
年卷期: 2006 年 149 卷 1-2 期
页码:
收录情况: SCI
摘要: A partial amphiploid, TE-3, between Triticum aestivum cv. Chinese Spring (CS) and Thinopyrum intermedium ssp. trichophorum was characterized by cytological observation, genomic in situ hybridization (GISH), seed storage protein electrophoresis and disease resistance screening. The TE-3 plants were deeply covered with pubescence, which is characteristic of the Th. intermedium ssp. trichophorum parent. Feulgen staining of the somatic metaphases revealed that the chromosome number varied from 52 to 56. TE-3 pollen mother cells (PMCs) regularly showed two to four univalents and 25 to 27 bivalents, indicating a degree of cytological instability. Giemsa-C banding showed that the Thinopyrum chromosomes in TE-3 produced strong heterochromatin bands. GISH analysis suggested that the alien chromosomes in TE-3 consisted of eight St chromosomes, four J(s) chromosomes, and two J genome chromosomes, as well as two St-J translocation chromosomes. Seeds storage proteins separated by acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) showed that TE-3 expressed some of Th. intermedium ssp. trichophorum specific gliadin and glutenin bands. When inoculated with stripe rust and powdery mildew isolates, TE-3 expressed resistance derived from its Thinopyrum parent. It appears that TE-3 can be used as a donor source in wheat breeding programs to introduce novel variation for quality and disease resistance.
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