您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A-type ECG and EGCG dimers inhibit 3T3-L1 differentiation by binding to cholesterol in lipid rafts

文献类型： 外文期刊

作者： Zhu, Wei 1 ; Deng, Xiangyi 1 ; Peng, Jinming 1 ; Zou, Bo 3 ; Li, Chunmei 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China

2.Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Food Sci, Wuhan, Hubei, Peoples R China

3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou, Guangdong, Peoples R China

关键词： A-type ECG and EGCG dimers;Cholesterol;Disruption;Lipid rafts;3T3-L1 preadipocytes differentiation

期刊名称：JOURNAL OF NUTRITIONAL BIOCHEMISTRY （ 影响因子：6.048； 五年影响因子：6.114 ）

ISSN： 0955-2863

年卷期： 2017 年 48 卷

页码：

收录情况： SCI

摘要： The present study aimed to explore the underlying mechanisms of epicatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epicatechin-3-gallate (A-type ECG dimer) and epigallocatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epigallocatechin-3-gallate (A-type EGCG dimer) involved in their strong inhibitory effects on 3T3-L1 preadipocytes differentiation. In the synthetic "lipid raft-like" liposome, A-type ECG and EGCG dimers incorporated into the liposome with high affinity and decreased the fluidity of the liposome. In 3T3-L1 preadipocytes, A-type ECG and EGCG dimers possibly bonded to lipid rafts cholesterol and disrupted the integrity of lipid rafts, thus exerting their notable inhibitory effects on 3T3-L1 preadipocytes differentiation by suppressing mitotic clonal expansion process and mRNA levels of PPAR gamma, C/EBP alpha, and SREBP1C. A highly positive correlation between the cholesterol binding capacity of the two dimers and their inhibitory effect on 3T3-L1 preadipocytes differentiation (R-2=0.9328) was observed. Molecular dynamics simulation further verified that A-type ECG and EGCG dimers could bond to cholesterol via hydrogen bonding. The results of this study suggested that the disruption of A-type ECG and EGCG dimers on membrane lipid rafts by targeting cholesterol in the lipid rafts was involved in the underlying mechanisms of their strong inhibitory effects on 3T3-L1 preadipocytes differentiation. This broadens the understanding of the molecular mechanisms of polyphenols on modulating and controlling of metabolic dysregulation, particularly adipocyte differentiation, which is a significant risk factor associated with the development of cardiovascular disease. (C) 2017 Elsevier Inc. All rights reserved.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台