您好,欢迎访问上海市农业科学院 机构知识库!

The mechanism of polysaccharide synthesis of Sanghuangporus sanghuang based on multi-omic analyses and feedback inhibition

文献类型: 外文期刊

作者: Li, Tingting 1 ; Wang, Qin 1 ; Yang, Yan 3 ; Song, Dingka 4 ;

作者机构: 1.Shanghai Univ Med & Hlth Sci, Affiliated Zhoupu Hosp, Shanghai, Peoples R China

2.Shanghai Univ Med & Hlth Sci, Shanghai, Peoples R China

3.Shanghai Acad Agr Sci, Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Inst Edible Fungi,Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China

4.Shanghai Jiao Tong Univ, Ctr Single Cell Om, Sch Publ Hlth, State Key Lab Oncogenes & Related Genes,Sch Med, Shanghai 200025, Peoples R China

关键词: S. sanghuang; Polysaccharide; Structural characterization; Comparative multi-omics; Integrative analysis

期刊名称:CARBOHYDRATE POLYMERS ( 影响因子:11.2; 五年影响因子:10.2 )

ISSN: 0144-8617

年卷期: 2023 年 321 卷

页码:

收录情况: SCI

摘要: S. sanghuang polysaccharide has various biological roles in promoting human health, however, the underlying mechanism of polysaccharide synthesis in S. sanghuang remain elusive. In the present study, the molecular structure of novel polysaccharide in the mutant S. sanghuang strain A130 with high yield of polysaccharide was characterized. The critical genes/proteins and pathways involved in polysaccharide synthesis were investigated via comparative transcriptomic, proteomic, and integrative analysis between wildtype strain SH-1 and A130. An integrated analysis of transcriptomic and proteomic results was also performed to locate potential regulators in the production of polysaccharides. The genes of cellobiohydrolase1 (CBH1) and MutS Homolog 6 (MSH6) related to glycolysis/gluconeogenesis were differentially expressed between A130 and SH-1, suggesting the potential involvement of these genes in regulating the production of polysaccharide. Proteomic analysis revealed that the abundance of Tyrosinase (TYR) and Trehalase (TREH) were substantially different between A130 and SH-1. The potential involvement of TYR in polysaccharide production was confirmed by transcriptomic-proteomic integrated analysis. The biological role of TYR and TREH in polysaccharide production was further verified by feedback inhibition of kojic acid and validamycin A, respectively. Overall, our study provides critical insights for the polysaccharide synthesis and high yield of polysaccharide through genes/pathways regulating in S. sanghuang.

  • 相关文献
作者其他论文 更多>>