MiR-29b inhibits COC expansion and oocyte in vitro maturation via induction of ROS by targeting CYCS
文献类型： 外文期刊
作者： Zhan, Xiaoshu 1 ; Qi, Nanshan 2 ; Toms, Derek 4 ; Freiburger, Renee 2 ; Fletcher, Lauren 2 ; Wang, Bingyun 1 ; Li, Julang 2 ;
作者机构： 1.Foshan Univ, Sch Anim Sci & Technol, Foshan 528231, Guangdong, Peoples R China
2.Univ Guelph, Dept Anim Biosci, Guelph, ON N1G 2W1, Canada
3.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China
4.Sunnybrook Res Inst, Toronto, ON, Canada
关键词： MiRNAs; Oocyte maturation; ROS; COC expansion
期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：3.3； 五年影响因子：2.6 ）
ISSN： 0378-4320
年卷期： 2024 年 270 卷
页码：
收录情况： SCI
摘要： Cumulus-oocyte complex (COC) expansion and oocyte maturation are crucial processes for embryo development and fertility across species. Although miR-29b has been detected in porcine ovarian granulosa cells, its specific role in regulating oocyte maturation remains largely unknown. In this study, using the pig as a model, we report that over-expression of miR-29b lead to a decrease of COC expansion area and inhibits oocyte maturation (P<0.05). P <0.05). This suppression correlated with a decrease expression of COC-expansion-associated genes, including SHAS2, , ADAMTS1, , ADAMTS2, , ADAMTS17 and PTX 3 in both mural granulosa cells (mGCs) and cumulus granulosa cells (cGCs). Further investigation revealed that miR-29b over-expression induces reactive oxygen species (ROS) accumulation in both mGCs and cGCs, conversely, knock-down of miR-29b reverses all these effects. Treatment with the antioxidant beta- mercaptoethanol alleviates ROS accumulation, rescues COC expansion and restores oocyte polar body formation impaired by miR-29b mimics. Computational analysis predicted CYCS, , the gene encoding cytochrome C, as a potential target of miR-29b. Subsequent examination demonstrated that miR-29b downregulates CYCS at both mRNA and protein levels. Dual-luciferase reporter assays further confirmed that miR-29b interacts with the 3'-untranslated region (3'UTR) of CYCS. Over-expression of CYCS decreases ROS accumulation and promotes COC expansion (P<0.05). P <0.05). These results indicate that miR-29b regulates COC expansion and oocyte maturation in vitro by inducing ROS, likely through targeting of CYCS. This study sheds light on the role of miR-29b in oocyte maturation and provides insight into the regulatory function of miRNAs in ovarian physiology.
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