Evaluation of Germplasm and Development of Markers for Resistance to Plasmodiophora brassicae in Radish (Raphanus sativus L.)
文献类型： 外文期刊
作者： Wang, Qingbiao 1 ; Wang, Yanping 1 ; Qian, Huihui 1 ; Zhang, Ziye 1 ; Zhang, Li 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China
2.Minist Agr & Rural Affairs Peoples Republ China, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China
3.Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China
关键词： radish germplasm; clubroot resistance; Plasmodiophora brassicae; CRa; marker-assisted selection
期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）
ISSN：
年卷期： 2022 年 12 卷 3 期
页码：
收录情况： SCI
摘要： The rapid spread of clubroot disease caused by Plasmodiophora brassicae threatens radish (Raphanus sativus) production in China because some cultivation types lack clubroot-resistant (CR) genes. However, few molecular markers for clubroot resistance have been developed and used in hybrid breeding programs. In this study, 27 immune and 6 highly resistant accessions were identified among 95 radish inbred lines. The genes Rsa10003637 and Rsa10025569 / Rsa10025571 were respectively identified from an XYB36-2 reference genome as the homologs of Crr1 and CRa from Brassica raga by means of homology and synteny analysis. The association between the degree of clubroot resistance and the genotype of these CR genes suggested that Rsa10025569 H3 can be used as a clubroot-resistant haplotype. The sequence identity of Rsa10025569 in clubroot-resistant lines (CR-60 and CR-88) and clubroot-susceptible lines (CR-10 and CR-35) was 92.47%, and there was a 699 bp insertion at the end of the fourth exon in the clubroot-susceptible line. Association analysis of a BC1F1 population derived from the cross CR-88 (resistance) x CR-10 (susceptible) revealed an apparent correlation between polymorphisms at the Rsa10025569 locus and degree of clubroot resistance. On the basis of the results, molecular marker-assisted selection was used to transfer disease resistance genes to susceptible varieties and a new CR germplasm of Xinlimei was obtained.
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