Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice
文献类型： 外文期刊
作者： Min, Ting 1 ; Sun, Jie 1 ; Yi, Yang 1 ; Wang, Hong-Xun 1 ; Hang, Fei 3 ; Ai, You-Wei 1 ; Wang, Li-Mei 1 ;
作者机构： 1.Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China
2.Hubei Collaborat Innovat Ctr Proc Agr Prod, Wuhan 430023, Peoples R China
3.Minist Agr, Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Key Lab Funct Foods, Guangzhou 510610, Guangdong, Peoples R China
关键词： longan pulp;polysaccharide-protein complex;HPSEC-FD;pharmacokinetics;distribution
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN： 1422-0067
年卷期： 2015 年 16 卷 10 期
页码：
收录情况： SCI
摘要： A high performance size exclusion-fluorescence detection (HPSEC-FD) method combined with fluorescein isothiocyanate (FITC) prelabeling was established for the microanalysis of polysaccharide-protein complexes from longan pulp (LPP). FITC-labeled LPP (LPPF) was fractionated by gel filtration chromatography. The weight-average molecular weight and FITC substitution degree of LPPF were 39.01 kDa and 0.20%, respectively. The HPSEC-FD calibration curves linear over the range of 1-200 mu g/mL in mouse plasma, spleen and lung samples with correlation coefficients greater than 0.995. The inter-day and intra-day precisions of the method were not more than 6.9%, and the relative recovery ranged from 93.7% to 106.4%. The concentration-time curve of LPPF in plasma following intravenous (i.v.) administration at 40 mg/kg body weight well fitted to a two-compartment model. LPPF rapidly eliminated from plasma according to the short half-lives (t(1/2) = 2.23 min, t(1/2) = 39.11 min) and mean retention times (MRT0-t = 1.15 h, MRT0- = 1.39 h). After administration over 5 to 360 min, the concentration of LPPF in spleen homogenate decreased from 7.41 to 3.68 mu g/mL; the concentration in lung homogenate decreased from 9.08 to 3.40 mu g/mL. On the other hand, the increasing concentration of LPPF fraction with low molecular weight in heart homogenate was observed.
- 相关文献
作者其他论文 更多>>
-
Effects of different oil fractions and tannic acid concentrations on konjac glucomannan-stabilized emulsions
作者：Long, Min;Ren, Yuanyuan;Li, Zhenshun;Yin, Chaomin;Sun, Jie
关键词：Konjac glucomannan; Tannic acid; Hydrogen bonds; High internal phase emulsion; Interfacial crosslinking
-
Regulations and mechanisms of 1-methylcyclopropene treatment on browning and quality of fresh-cut lotus (Nelumbo nucifera Gaertn.) root slices
作者：Chen, Jinhui;Xu, Yuhan;Yi, Yang;Hou, Wenfu;Wang, Limei;Ai, Youwei;Wang, Hongxun;Min, Ting;Yi, Yang;Hou, Wenfu;Wang, Hongxun;Min, Ting
关键词：1-MCP; Enzymatic browning; Phenylpropane metabolism; Ethylene biosynthesis; Ethylene signal transduction
-
Effects of Vacuum Packaging on Enzymatic Browning and Ethylene Response Factor (ERF) Gene Expression of Fresh-cut Lotus Root
作者：Min, Ting;Liu, En-chao;Xie, Jun;Yi, Yang;Wang, Li-mei;Ai, You-wei;Wang, Hong-xun;Yi, Yang;Wang, Hong-xun
关键词：Nelumbo nucifera; browning; PAL; PPO; POD; ERF
-
Effect of mild heat treatment on shelf life of fresh lotus root
作者：Li, Shuyi;He, Xu;Liu, Zhiwei;Yi, Yang;Wang, Hongxun;Lamikanra, Olusola;Li, Xiaojin;Liu, Zhiwei;Yi, Yang;Wang, Hongxun
关键词：Lotus root;Physical treatment;Texture;Protein;Postharvest life
-
Characterization of polysaccharide from longan pulp as the macrophage stimulator
作者：Yi, Yang;Wang, Hongxun;Min, Ting;Yi, Yang;Wang, Hongxun;Zhang, Ruifen;Huang, Fei;Liu, Lei;Zhang, Mingwei
关键词：
-
The effect of different temperatures on browning incidence and phenol compound metabolism in fresh-cut lotus (Nelumbo nucifera G.) root
作者：Min, Ting;Xie, Jun;Zheng, Menglin;Yi, Yang;Hou, Wenfu;Wang, Limei;Ai, Youwei;Wang, Hongxun;Yi, Yang;Hou, Wenfu;Wang, Hongxun
关键词：PAL;PPO;POD;Enzyme activity;Gene expression
