文献类型： 外文期刊
作者： Yu, Xuekui 1 ; Jiang, Jiansen 1 ; Sun, Jingchen 1 ; Zhou, Z. Hong 1 ;
作者机构： 1.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90089 USA
2.Univ Calif Los Angeles, Calif Nanosyst Inst, Los Angeles, CA USA
3.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Subtrop Sericulture & Mulberry Resources Protect, Guangzhou, Guangdong, Peoples R China
期刊名称：ELIFE （ 影响因子：8.14； 五年影响因子：9.056 ）
ISSN： 2050-084X
年卷期： 2015 年 4 卷
页码：
收录情况： SCI
摘要： mRNA transcription in dsRNA viruses is a highly regulated process but the mechanism of this regulation is not known. Here, by nucleoside triphosphatase (NTPase) assay and comparisons of six high-resolution (2.9-3.1 angstrom) cryo-electron microscopy structures of cytoplasmic polyhedrosis virus with bound ligands, we show that the large sub-domain of the guanylyltransferase (GTase) domain of the turret protein (TP) also has an ATP-binding site and is likely an ATPase. S-adenosyl-L-methionine (SAM) acts as a signal and binds the methylase-2 domain of TP to induce conformational change of the viral capsid, which in turn activates the putative ATPase. ATP binding/hydrolysis leads to an enlarged capsid for efficient mRNA synthesis, an open GTase domain for His217-mediated guanylyl transfer, and an open methylase-1 domain for SAM binding and methyl transfer. Taken together, our data support a role of the putative ATPase in mediating the activation of mRNA transcription and capping within the confines of the virus.
- 相关文献
作者其他论文 更多>>
-
Single-nucleus RNA sequencing reveals midgut cellular heterogeneity and transcriptional profiles in Bombyx mori cytoplasmic polyhedrosis virus infection
作者：Fei, Shigang;Awais, Mian Muhammad;Zou, Jinglei;Xia, Junming;Wang, Yeyuan;Kong, Yibing;Feng, Min;Sun, Jingchen
关键词：Bombyx mori; BmCPV; midgut; single-nucleus RNA sequencing
-
Single-Nucleus Sequencing of Fat Body Reveals Distinct Metabolic and Immune Response Landscapes in Silkworm Larvae after Bombyx mori Nucleopolyhedrovirus Infection
作者：Feng, Min;Xia, Junming;Fei, Shigang;Huang, Yigui;Lai, Wenxuan;Sun, Jingchen;Swevers, Luc
关键词：
-
Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles
作者：Feng, Min;Sun, Jingchen;Swevers, Luc
关键词：scRNA-seq; silkworm; hemocyte; marker gene; Bombyx mori
-
Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation
作者：Wang, Xiong;Ma, Guangyu;Ren, Feifei;Awais, Mian Muhammad;Sun, Jingchen
关键词：Bombyx mori; nuclear polyhedrosis virus; viral proliferation; co-immunoprecipitation
-
Impact of Group II Baculovirus IAPs on Virus-Induced Apoptosis in Insect Cells
作者：Sun, Jingchen
关键词：insect cell; group II baculovirus; inhibitor of apoptosis protein; apoptosis
-
Impact of Molecular Modification on the Efficiency of Recombinant Baculovirus Vector Invasion to Mammalian Cells and Its Immunogenicity in Mice
作者：Zheng, Hao;Pan, Yong;Wang, Xiong;Tian, Weibin;Sun, Jingchen;Yao, Lunguang
关键词：baculovirus display system; envelope protein; high-efficiency display; multiple vaccine
-
Multiple conformations of trimeric spikes visualized on a non-enveloped virus
作者：Zhang, Yinong;Sun, Jingchen;Zhang, Yinong;Cui, Yanxiang;Zhou, Z. Hong;Zhang, Yinong;Sun, Jingchen;Zhou, Z. Hong
关键词：
