您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evaluation of three fluorochromes as in situ growth markers in Manila clam Ruditapes philippinarum

文献类型： 外文期刊

作者： Wu, Lei 1 ; Zhou, Liqing 1 ; Zhang, Tianshi 1 ; Li, Li 3 ; Xu, Xinghong 2 ; Wu, Biao 1 ; Liu, Zhihong 1 ; Sun, Xiujun 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Pilot Natl Lab Marine Sci & Technol Qingdao,Key La, Qingdao 266071, Peoples R China

2.Jiangsu Ocean Univ, Lianyungang 222005, Peoples R China

3.Natl Oceanog Ctr, Marine Sci Res Inst Shandong Prov, Qingdao 266104, Peoples R China

4.Yellow Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Qingdao 266071, Peoples R China

关键词： Aquaculture bivalve; Calcein; Alizarin red S; Oxytetracycline; Fluorescent marking

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2023 年 28 卷

页码：

收录情况： SCI

摘要： Marking technology is essential to evaluate the growth performance of shellfish species in aquaculture. Some fluorochromes have been widely used in shellfish growth studies, but their marking quality remains unresolved. In order to establish an effective marking technology in bivalves, three fluorochromes are used to evaluate the suitability of in situ growth markers in spats of commercially important bivalve, Manila clam Ruditapes philippinarum. Three different concentrations of calcein (CAL; 25 mg/L, 50 mg/L and 100 mg/L), alizarin red S (ARS; 5 mg/L, 10 mg/L and 20 mg/L) and oxytetracycline hydrochloride (OTC; 250 mg/L, 500 mg/L and 750 mg/L) were used to examine their potential effects on marker quality, growth and survival of spats. In general, marking quality scores for the three fluorochromes have a positive correlation with their increased concentrations. CAL (50-100 mg/L) and ARS (10-20 mg/L) have produced healthy spats with a bright and clear fluorescent shell mark after the recovery culture for 69 days. In contrast, OTC appears to have the weak staining signals at all concentrations. The growth and survival rates affected by fluorochrome immersion are negligible at all concentrations. The present findings indicate that in situ CAL and ARS marking of spats with moderate concentrations are non-invasive and effective method for growth studies in clams. The suitability of CAL and ARS as in situ growth markers in clams will provide new marking techniques to distinguish cohorts or families in aquaculture.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台