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Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray

文献类型： 外文期刊

作者： Zhou, Gao-Feng 1 ; Liu, Yong-Zhong 1 ; Sheng, Ou 1 ; Wei, Qing-Jiang 1 ; Yang, Cheng-Quan 1 ; Peng, Shu-Ang 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China

2.Gannan Normal Univ, Coll Navel Orange, Natl Navel Orange Engn Res Ctr, Ganzhou, Peoples R China

3.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou, Guangdong, Peoples R China

4.Jiangxi Agr Univ, Coll Agr, Nanchang, Peoples R China

关键词： citrus rootstock;boron deficiency;root;gene expression;SSH;cDNA microarray

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2015 年 5 卷

页码：

收录情况： SCI

摘要： Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

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