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Ascorbate peroxidase from Jatropha curcas enhances salt tolerance in transgenic Arabidopsis

文献类型： 外文期刊

作者： Chen, Y. 1 ; Cai, J. 2 ; Yang, F. X. 3 ; Zhou, B. 1 ; Zhou, L. R. 1 ;

作者机构： 1.Sichuan Univ, Coll Architecture & Environm, Chengdu 610064, Sichuan, Peoples R China

2.Sichuan Univ, West China Sch Pharm, Chengdu 610064, Sichuan, Peoples R China

3.Guangdong Acad Agr Sci, Guangdong Key Lab Ornamental Plant Germplasm Inno, Environm Hort Res Inst, Guangzhou, Guangdong, Peoples R China

关键词： Oxidative stress;Ascorbate peroxidase;Salt stress;H2O2;Jatropha curcas

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Ascorbate peroxidase (APX) plays a central role in the ascorbate-glutathione cycle and is a key enzyme in cellular H2O2 metabolism. It includes a family of isoenzymes with different characteristics, which are identified in many higher plants. In the present study, we isolated the APX gene from Jatropha curcas L, which is similar with other previously characterized APXs as revealed by alignment and phylogenetic analysis of its deduced amino acid sequence. Real-time qPCR analysis showed that the expression level of JcAPX transcript significantly increased under NaCl stress. Subsequently, to elucidate the contribution of JcAPX to the protection against salt-induced oxidative stress, the expression construct p35S: JcAPX was created and transformed into Arabidopsis and transcribed. Under 150-mM NaCl stress, compared with wild type (WT), the overexpression of JcAPX in Arabidopsis increased the germination rate, the number of leaves, and the rosette area. In addition, the transgenic plants had longer roots, higher total chlorophyll content, higher total APX activity, and lower H2O2 content than the WT under NaCl stress conditions. These results suggested that higher APX activity in transgenic lines increases the salt tolerance by enhancing scavenging capacity for reactive oxygen species under NaCl stress conditions.

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