文献类型： 外文期刊

作者： Li Peiqian ¹ ; Pu Xiaoming ¹ ; Shen Huifang ² ; Zhang Jingxin ² ; Huang Ning ¹ ; Lin Birun ¹ ;

作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

2.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab New Tech Plant Protect Guangdong, Guangzhou 510640, Guangdong, Peoples R China

关键词： Fusarium wilt;plant pathogen;XTT;CSLM

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN： 0378-1097

年卷期： 2014 年 350 卷 2 期

页码：

收录情况： SCI

摘要： To the authors' knowledge, most studies on biofilm formation have focused on bacteria and yeasts. So far, biofilm formation by fungal plant pathogen has not been reported. In this study, the biofilm-forming capacity of Fusarium oxysporum f.sp. cucumerinum was evaluated. For biofilm quantification, a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium-hydroxide (XTT) reduction assay was used to observe metabolic activity. Fluorescence and confocal scanning laser microscopy revealed that the biofilms have a highly heterogeneous architecture composed of robust hyphae and extracellular polysaccharide materials. Additionally, the influence of physical factors on F.oxysporum biofilm formation and the susceptibility of biofilms to environmental stress was investigated. Biofilms were less susceptible to heat, cold, UV light and three fungicides than were their planktonic counterparts. Our findings may provide a novel perspective on the pathogenic mechanism associated with biofilms of F.oxysporum f. sp. cucumerinum.