您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Bioassay- and liquid chromatography/mass spectrometry-guided acetylcholinesterase inhibitors from Picriafel-terrae

文献类型： 外文期刊

作者： Wen, Lu 1 ; Wei, Qiqiu 1 ; Chen, Gang 1 ; Liu, Fan 3 ; Zhang, Shichang 1 ; You, Tinghuo 1 ;

作者机构： 1.Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou, Guangdong, Peoples R China

2.Guangdong Pharmaceut Univ, Inst Mat Med, Guangdong Prov Key Lab Adv Drug Delivery, Guangzhou, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Sericulture & Farm Produce Proc Res Inst, Guangzhou, Guangdong, Peoples R China

关键词： Acetylcholinesterase inhibitory activity;LC-bioassay-ESIMS;Picriafel-terrae

期刊名称：PHARMACOGNOSY MAGAZINE （ 影响因子：1.085； 五年影响因子：1.65 ）

ISSN： 0973-1296

年卷期： 2013 年 9 卷 36 期

页码：

收录情况： SCI

摘要： Background: Picria fel-terrae is a traditional Chinese medicine. Materials and Methods: A new approach to the search for acetylcholinesterase (AChE) inhibitors from Picria fel-terrae is presented. Results: Bioassay- and LC-MS-guided fractionation of the ethyl acetate extract was from traditional Chinese medicine P.fel-terrae. Following primary extraction, the ethyl acetate extracts fraction of P.fel-terrae showed strong AChE inhibitory activities. So the sample was separated using highperformance liquid chromatography (HPLC). The effluent was split towards two identical 96-well fraction collectors, and the presence of the biologically interesting portion and chromatographic fractions could be readily detected by analyzing selected ion chromatograms through an electrophoresis-electrospray ionization mass spectrometry (ESIMS) system for accurate mass measurement. One 96-well plate was used for a bioassay (AChE-inhibitory assay) and detected the bioactivity and position of the relevant peak in the chromatogram. The positive well in the second 96-well plate was used for identification by LC-() ESIMS. Conclusion: As abovementioned, the AChE inhibitory constituents from P.fel-terrae by LC-bioassay-ESIMS were rapid identified. Liquid chromatography/ mass spectrometry (LC-MS) screening detected the presence of six active compounds, identified as picfeltarraenin IA (1), picfeltarraenin IB (2), picfeltarraenin IV (3), picfeltarraenin X (4), picfeltarraenin XI (5), and one unknown compound. The structures were further determined by 13C NMR. The six compounds expressed stronger AChE inhibition than the known AChE inhibitorTacrine. Above all, the value of this LC-bioassay-ESIMS methodology is highlighted by the finding and structure elucidation of the active constituents from many other structural families of natural products.

  • 相关文献

[1]In vitro Antioxidant and Acetylcholinesterase Inhibitory Activities of the Sesquiterpenes of a Symbiotic Actinomycete Streptomyces sp from South China Sea. Wen, Lu,Chen, Gang,Zhang, Shichang,You, Tinghuo,Fu, Yuhong,Yao, Xiangcao,Chen, Gang,Liu, Fan. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台