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A New Diagnostic System for Detection of 'Candidatus Liberibacter asiaticus' Infection in Citrus

文献类型： 外文期刊

作者： Lu, Lianming 1 ; Cheng, Baoping 2 ; Yao, Jinai 3 ; Peng, Aitian 2 ; Du, Danchao 1 ; Fan, Guocheng 4 ; Hu, Xiurong 1 ; Zha 1 ;

作者机构： 1.Zhejiang Citrus Res Inst, Taizhou 318020, Peoples R China

2.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Guangdong, Peoples R China

3.Fujian Acad Agr Sci, Plant Protect Res Inst, Fuzhou 350013, Peoples R China

4.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2013 年 97 卷 10 期

页码：

收录情况： SCI

摘要： In this study, two polyclonal antibodies were produced against the Omp protein of 'Candidatus Liberibacter asiaticus'. First, omp genes were sequenced to exhibit 99.9% identity among 137 isolates collected from different geographical origins. Then, two peptides containing the hydrophobic polypeptide-transport-associated (POTRA) domain and beta-barrel domain, respectively, were identified on Omp protein. After that, these two peptides were overexpressed in Escherichia coli and purified by affinity chromatography to immunize the white rabbits. Finally, the antiserum was purified by affinity chromatography. The two Omp antibodies gave positive results (0.454 to 0.633, 1:1,600 dilution) in enzyme-linked immunosorbent assay against 'Ca. L. asiaticus' infected samples collected from different geographical origins but revealed negative results against other pathogen-infected, nutrient-deficient and healthy samples. The antibody against the POTRA domain of Omp protein could detect 'Ca. L. asiaticus' in 45.7% of the symptomatic samples compared with a 56.2% detection rate with a polymerase chain reaction assay. These new antibodies will provide a very useful supplement to the current approaches to 'Ca. L. asiaticus' detection and also provide powerful research tools for tracking distribution of this pathogen in vivo.

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