文献类型： 外文期刊
作者： Zhang Li 1 ; Xie Xiu-juan 2 ; Jia Shan-gang 3 ; Xiao Mei 1 ; Lin Shu-dai 2 ; An Li-long 1 ; Luo Wen 2 ; Jia Xin-zheng 2 ; Ni 1 ;
作者机构： 1.Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China
2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
3.Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China
关键词： Peking duck;miRNA star species (miRNA*);skin;nucleotide bias
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2013 年 12 卷 9 期
页码：
收录情况： SCI
摘要： A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3'-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the "seed region". miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.
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