Biochemical Characterization of the Outer Membrane Enzyme OMPLA from Riemerella anatipestifer
文献类型： 外文期刊
作者： Liao, Shenquan 1 ; Yuan, Jianfeng 1 ; Qin, Zonghua 1 ; Wu, Caiyan 1 ; Lv, Minna 1 ; Qi, Nanshan 1 ; Cai, Jianping 1 ; Sun 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Vet Med, Guangzhou 510642, Guangdong, Peoples R China
关键词： Riemerella anatipestifer;outer membrane phospholipase A;methyl arachidonyl fluorophosphonate;protein;database
期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）
ISSN： 1680-5593
年卷期： 2012 年 11 卷 21 期
页码：
收录情况： SCI
摘要： A gene (pld4) encoding a 33.0 kDa protein. with significant homology to the Escherichia coli outer membrane phospholipase A was identified from Riemerella anatipestifer genome database. The results of this study revealed that this gene encoded an Outer Membrane Phospholipase A (OMPLA) in Riemerella anatipestifer. The recombinant ONEPLA protein. was expressed and its general biochemical features were also, determined. Methyl Arachidonyl Fluorophosphonate (MAFP) could specifically inhibit the OMPLA activity. These observations suggest that OMPLA could be exploited as a drug target in the bacteria and MAFP may serve as a lead compound for drug discovery against R. anatipestifer.
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