文献类型: 外文期刊
作者: Li, Yanyan 1 ; Li, Zhiliang 1 ; Li, Zhenxing 1 ; Luo, Shaobo 1 ; Sun, Baojuan 1 ;
作者机构: 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Guangdong, Peoples R China
2.S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China
关键词: Eggplant;heat stress;cDNA amplified fragment length polymorphism (cDNA-AFLP);transcript-derived fragment (TDF);quantitative real-time polymerase chain reaction (Q-PCR)
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 79 期
页码:
收录情况: SCI
摘要: In order to identify differentially expressed genes involved in heat shock response, cDNA amplified fragment length polymorphism (cDNA-AFLP) and quantitative real-time polymerase chain reaction (Q-PCR) were used to study gene expression of eggplant seedlings subjected to 0, 6 and 12 h at 43 degrees C. A total of 53 of over 2400 bands amplified were differentially expressed between the control and heat treatment; 42 up-regulated and 11 corresponding to genes repressed by heat stress. 24 transcript-derived fragments (TDFs) were successfully isolated, cloned and sequenced. BLAST searching revealed that 15 heat stress response transcripts presented similarities with those that encoded small molecular shock protein, disease resistance protein, stress-related protein, enzymes related to photosynthesis as well as biosynthesis of protein, and so on. The expression patterns of five randomly selected TDFs were confirmed by using Q-PCR experiments. The direct and indirect relationships of genes corresponding to the 15 TDFs with stress tolerance are discussed.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification and Expression Analysis of the SPL Gene Family in Solanum melongena (Eggplant)
作者:Cheng, Hefen;Zhou, Dinggang;Cheng, Hefen;Li, Zhiliang;Sun, Baojuan;Zhao, Wei;Zhang, Songyuan;Li, Tao;You, Qian
关键词:eggplant;
SmSPL ; genome; fruit development; expression patterns -
A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt
作者:Gong, Chao;Li, Zhiliang;Sun, Baojuan;Luo, Wenlong;Luo, Shanwei;Chen, Shuting;Mai, Peiting;Li, Zhenxing;Li, Tao;Wang, Zhenshuo;Li, Ye;Wang, Yikui
关键词:
-
Genetic diversity and population structure analysis of 418 tomato cultivars based on single nucleotide polymorphism markers
作者:Xu, Weijie;Gong, Chao;Mai, Peiting;Li, Zhenxing;Sun, Baojuan;Li, Tao
关键词:genetic relationship; population structure; SNPs; DNA fingerprint; SNP markers
-
Characterization of odor-contributing volatile in Capsicum chinense 'JT-1'fruits during development and transcriptome analysis of key fruit-aroma formation periods
作者:Heng, Zhou;Xu, Xiaowan;Xu, Xiaomei;Wang, Hengming;Li, Zhenxing;Li, Zhiliang;You, Qian;Sun, Baojuan;Gong, Chao;Yin, Yan;Li, Ying;Li, Tao;Liu, Lei;Li, Ying;Li, Tao
关键词:Chili pepper; GC-O-MS; Volatile organic compounds; Transcriptome
-
Genome-Wide Analysis Revealed NBS-LRR Gene Candidates Associated with Bacterial Wilt Resistance in Eggplant (Solanum melongena L.)
作者:Jiang, Yaolan;Cheng, Hefen;Zhou, Dinggang;Jiang, Yaolan;Li, Zhiliang;Cheng, Hefen;Zhao, Wei;Li, Tao;Sun, Baojuan;You, Qian;Li, Wenxiang
关键词:eggplant; NBS-LRR; disease resistance; genome-wide investigation; bacterial wilt resistance
-
Enhanced Valsa canker resistance conferred by expression of MdLecRK-S.4.3 in Pyrus betulifolia is largely suppressed by PbePUB36
作者:Sun, E.;Yu, Hongqiang;Cai, Minrui;Mao, Xia;Li, Yanyan;Zuo, Cunwu;Chen, Zhongjian;Zuo, Cunwu
关键词:Apple; co-expression analysis; gene duplication; L-type lectin receptor-like kinases; Malus domestica; pattern recognition receptors; pear; Pyrus betulifolia; reciprocal confrontation role; Valsa canker
-
Mapping and validation of the epistatic D and P genes controlling anthocyanin biosynthesis in the peel of eggplant (Solanum melongena L.) fruit
作者:You, Qian;Li, Huimin;Wu, Jun;Li, Tao;Li, Zhiliang;Sun, Baojuan;Li, Huimin;Wu, Jun;Sun, Guangwen;Wang, Yikui;Li, Huimin
关键词:



