Phylogenetic analysis of the citrus Huanglongbing (HLB) bacterium based on the sequences of 16S rDNA and 16S/23S rDNA intergenic regions among isolates in China
文献类型： 外文期刊
作者： Ding, Fang 1 ; Deng, Xiuxin 2 ; Hong, Ni 1 ; Zhong, Yun 3 ; Wang, Guoping 1 ; Yi, Ganjun 3 ;
作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
3.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China
关键词： Citrus Huanglongbing;16S rDNA;16S/23S rDNA;Phylogenetic analysis
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）
ISSN： 0929-1873
年卷期： 2009 年 124 卷 3 期
页码：
收录情况： SCI
摘要： Phylogenetic analysis of Chinese isolates of the citrus Huanglongbing (HLB) bacterium based on the 16S rDNA and 16S/23S rDNA intergenic regions sequences was carried out. Nine HLB samples collected from different hosts with different symptoms in seven Chinese provinces, were subjected to PCR for amplifying and sequencing the 16S rDNA. The identity level among Chinese isolates was 98.5% to 100% and was the same with the Indian HLB isolate 'Poona' (GenBank accession number: L22532). By contrast, identity values were 97.5% to 97.8% with Candidatus Liberibacter africanus strain 'Nelspruit' (L22533), 96.3% to 97.3% with Ca. L. africanus subsp. 'Capensis' (AF137368), 95.3% to 96.5% with the Ca. Liberibacter sp. 'LSg2' (AY919312), and 94.9% to 96.0% with a strain of Ca. L. americanus from Brazil (SA o pound Paulo State; AY742824). A phylogenetic tree constructed with 16S rDNA sequences showed that all Chinese isolates belong to Ca. L. asiaticum. Analysis of the 16S/23S rDNA intergenic region was conducted on 18 HLB-diseased citrus samples with different symptoms, collected in seven provinces. These isolates showed no obvious variation and had an identity level > 99.0% with one another. Sequence analysis of 16S/23S rDNA intergenic region and the relative phylogenetic tree showed that the Chinese isolates are very close to Ca. L. asiaticus, and distinct from Ca. L. africanus and Ca. L. americanus. These results suggest that the Chinese HLB isolates belong to the species Candidatus Liberibacter asiaticus. This is the first report on the classification of HLB isolates from China based on molecular investigations.
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