文献类型： 外文期刊

作者： Jiwu, Zeng ¹ ; Ganjun, Yi ¹ ; Qiuming, Zhang ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Fruit Res Inst, Guangzhou 510640, Peoples R China

关键词： Carica papaya L.;vitrification

期刊名称：PROCEEDINGS OF THE SECOND INTERNATIONAL SYMPOSIUM ON PLANT GENETIC RESOURCES OF HORTICULTURAL CROPS, VOLS 1 AND 2

ISSN： 0567-7572

年卷期： 2007 年 760 期

页码：

收录情况： SCI

摘要： Papaya (Carica papaya L.) is an important fruit species and is widely cultivated in many tropical and subtropical countries. The conventional methods of maintaining papaya germplasm under nursery and orchard conditions require extensive space and labor. It is very difficult to conserve papaya germplasm by conventional methods. In this study shoot tips from papaya were multiplied in vitro and cryopreserved by vitrification. Micropropagation was improved by testing growth regulator concentrations. Shoots grown on MS medium with 0.5 mg/L BA, 0.1 mg/L NAA and 1.0 mg/L GA(3) were significantly better than on other treatments. Optimized vitrification procedures indicated that for papaya the best protocol was with shoot tips (2-3 mm) precultured for 3 days at 25 degrees CV on MS medium with 5% DMSO and 5% sucrose. Shoot tips (1.5 similar to 2.5 mm) were pretreated in 60% PVS2 for 50 min at room temperature, then they were completely dehydrated with 100% PVS2 for 30 min at 0 degrees C. After changing the solution with fresh PVS2 the materials were plunged directly into liquid nitrogen and stored for at least 24 hours. Shoot tips were rapidly warmed in a 40 degrees C water bath, and washed twice with 1.2 M sucrose solution and transferred to the optimized medium. Shoot tip regrowth was 52.6%. The shoots developed normally, rooted and survived transplantation.