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An amplified AuNP-mediated chemiluminescence resonance energy transfer aptasensor based on Nb.BbvCI-powered DNA walker for detecting ochratoxin A in foods

文献类型： 外文期刊

作者： Tang, Jidong 1 ; Wang, Ling 3 ; Ma, Wenjin 4 ; Fu, Ping 5 ; Zhang, Yanlin 6 ; Li, Xiaoqiong 1 ; Tao, Xiaoqi 3 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Peoples R China

3.Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China

4.Southwest Univ, Coll Vet Med, Joint Int Res Lab Anim Hlth & Anim Food Safety, Chongqing 400715, Peoples R China

5.Chinese Acad Med Sci & Peking Union Med Coll, Inst Blood Transfus, Chengdu 610052, Peoples R China

6.Zhoushan Inst Food & Drug Control, 49 Honglu Rd, Zhoushan 316012, Peoples R China

关键词： Chemiluminescent resonance energy transfer; Nb.BbvCI; DNA walker; Ochratoxin A; Aptasensor

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）

ISSN： 0026-265X

年卷期： 2025 年 208 卷

页码：

收录情况： SCI

摘要： The application of the fluorescence resonance energy transfer (FRET) strategy in the detection of ochratoxin A (OTA) is restricted by factors such as the photobleaching of fluorescent dyes, the autofluorescence of ochratoxin A (OTA), and the necessity for excitation by an external light source. The chemiluminescence resonance energy transfer (CRET) technique provides a more effective solution to this issue. In this paper, we developed a novel CRET aptasensor, based on gold nanoparticle (AuNP)-mediated CRET and Nb.BbvCI-powered DNA walker signal amplification. In the AuNPs/Capture-DNA/HRP probe, horseradish peroxidase (HRP) approached the AuNP surface, resulting in CRET. AuNPs quenched the chemiluminescence generated by HRP-catalyzed luminol. The presence of OTA induced the separation of the Walk-DNA from the aptamer and the formation of a double strand with Capture-DNA. This activated Nb.BbvCI to cleave Capture-DNA, releasing HRP and Walk-DNA. The WalkDNA acted as a DNA walker, moving toward the adjacent Capture-DNA and initiating the next cleavage by Nb.BbvCI. Ultimately, a significant amount of HRP was released from the AuNP surface, weakening the CRET effect while generating a strong chemiluminescent signal. The CRET aptasensor had a detection limit of 2.75 nM with a linear range of 5-40 nM and was applied to detect OTA in glutinous rice.

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