文献类型： 外文期刊

作者： Ding, F ¹ ; Wang, G ² ; Yi, G ² ; Zhong, Y ² ; Zeng, J ² ; Zhou, B ² ;

作者机构： 1.Huazhong Agr Univ, Plant Sci & Technol Acad, Wuhan 430070, Hubei, Peoples R China

2.Huazhong Agr Univ, Plant Sci & Technol Acad, Wuhan 430070, Hubei, Peoples R China; Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

关键词： Huanglongbing;greening;Candidatus Liberibacter asiaticus;citrus;lemon;wampee;diagnosis;single-step PCR;nested PCR

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）

ISSN： 1125-4653

年卷期： 2005 年 87 卷 3 期

页码：

收录情况： SCI

摘要： Single-step and nested polymerase chain reactions (PCR) were used to determine the presence of Candidatus Liberibacter asiaticus, the phloem-limited bacterial pathogen of Huanglongbing (HLB), in leaves of wampee [Clausena lansium (Lour.) Skeels], lemon [Citrus limon (L.) Burm.] and several other citrus species. Specific PCR products were obtained when single-step PCR and nested PCR were used to analyze wampee and lemon samples with or without visible HLB symptoms. Nested-PCR was found to be more accurate than single-step PCR with a sensitivity of about 104 times higher. The amount of bacterial DNA was positively correlated with HLB symptoms in the leaves. Amplicons from single-step PCR and those from nested PCR showed 100% and 99% identity, respectively, with sequences of the rplKAJL-rpoBC gene cluster of Ca. Liberibacter asiaticus. This pathogen is therefore able to infect wampee and lemon, in the leaves of which it can be readily detected by PCR. This is the first report of infections by Ca. L. asiaticus in wampee.