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Cloning of Etp28 gene of Eimeria tenella (Guangdong Strain) sporozoites and its expression in Baculovirus expression system

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文献类型：外文期刊

作者： Yang, L ¹ ; Zhu, W ² ; Wang, XZ ² ; Long, QX ² ; Xie, MQ ² ; Huang, XQ ² ;

作者机构： 1.Guangdong Acad Agr Sci, Guangzhou 510650, Peoples R China

2.Guangdong Acad Agr Sci, Guangzhou 510650, Peoples R China; Zhongshan Univ, State Key Lab Biol Control, Guangzhou 510275, Peoples R China; Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Peoples R China

关键词： Eimeria tenella;Etp28 gene;gene cloning;sequencing;baculovirus expression system

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （影响因子：3.848；五年影响因子：3.67 ）

ISSN： 1672-9145

年卷期： 1998 年 30 卷 3 期

页码：

收录情况： SCI

摘要： A refractile body antigen Etp28 gene of Eimeria tenella (Guangdong Strain) sporozoites was cloned by PCR from the synthesized first strand cDNA. It has a high homology with the previously reported Etp28 gene of Merck Strain LS18. The gene was expressed through standard procedures in the modified Autographa californica nuclear polyhedrosis virus (AcMNPV-OCC(-)) expression system and large amount of heterologous fusion protein (GST-6 X His-Etp28) was obtained. The expression product was about 21. 3% of the total cellular protein of an infected cell and corresponding to approximately 0.42 mg of recombinant protein per 10(6) cells. And the immunoprotective trial showed that this candidate for recombinant vaccine conferred partial protection against chicken coccidiosis.

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Cloning of Etp28 gene of Eimeria tenella (Guangdong Strain) sporozoites and its expression in Baculovirus expression system

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Cloning of Etp28 gene of Eimeria tenella (Guangdong Strain) sporozoites and its expression in Baculovirus expression system

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