文献类型： 外文期刊

作者： Li, Haonan ¹ ; Ackah, Michael ¹ ; Amoako, Frank Kwarteng ³ ; Asare, Aaron Tettey ³ ; Li, Jianbin ¹ ; Wang, Zhenjiang ⁵ ; Lin, Qiang ⁶ ; Qiu, Changyu ⁶ ; Zhao, Mengdi ⁷ ; Zhao, Weiguo ¹ ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericulture Biol & Biotechnol, Zhenjiang 212100, Peoples R China

2.Chinese Acad Agr Sci, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Sericultural Res Inst, Zhenjiang 212100, Peoples R China

3.Univ Cape Coast, Coll Agr & Nat Sci, Sch Biol Sci, Dept Mol Biol & Biotechnol, Cape Coast 00233, Ghana

4.Univ Kiel, Inst Plant Nutr & Soil Sci, Hermann Rodewald Str 2, D-24118 Kiel, Germany

5.Guangdong Acad Agr Sci, Sericulture & Agrifood Res Inst, Guangzhou 510610, Peoples R China

6.Sericulture Technol Promot Stn, Nanning 530007, Peoples R China

7.Suzhou Univ Sci & Technol, Dept Mat Sci & Engn, Suzhou 215011, Peoples R China

关键词： mulberry; Morus alba L.; stubble; targeted metabolomics; phytohormones; abscisic acid; gibberellin

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Vegetative propagation of mulberry (Morus alba L.) via sapling methods, due to the ability to exponentially multiply lateral buds on stem cuttings to enhance rapid shoot formation, is crucial for sericulture industries. The sprouting of mulberry using stubbles is an emerging method for rapid and mass production of mulberry leaves, but the growth mechanisms associated with its use remain obscure. This study is the first to report how the differential stubble lengths from mulberry plants alter and modulate phytohormones and the associated mechanisms. This study seeks to evaluate the growth mechanisms by elucidating the phytohormone signature modulation in response to differential stubble lengths of 0 cm, 5 cm, 10 cm, 20 cm, and a control via targeted metabolomics analysis in mulberry leaves. The results consistently show that the use of differential stubble lengths of mulberry promoted growth, the number of buds, aboveground biomass, and branch and leaf weights by improving the net photosynthesis, transpiration rate, stomatal conductance, and intercellular CO2 relative to the control. The differential stubble lengths not only caused contrasting responses in the contents of plant hormones, including salicylic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), jasmonic acid (JA), and gibberellin (GA), but also modulated higher elemental contents relative to the control. The results further reveal significant and positive correlations between the phytohormones and all growth, biomass, and photosynthetic parameters, highlighting the role of phytohormones in the sprouting and rejuvenation of mulberry stubbles. Meanwhile, the targeted metabolomics analysis identified a total of 11 differentially accumulated phytohormones in response to the differential stubble lengths, which were significantly implicated and enriched in three major pathways, including the biosynthesis of plant hormones (ko01070), metabolic pathways (ko01100), and the plant hormone signal transduction pathway (ko04575). The use of stubbles for rapid leaf production in mulberry plants is of great importance to improve early sprouting and cutting survival, as well as shortening growth and rooting time, and is highly recommended for the sericulture industries.