Functional characterization of tonoplast sugar transporter gene HpTST1 involved in soluble sugars accumulation during fruit development process of red pitaya (Hylocereus polyrhizus)
文献类型： 外文期刊
作者： Zheng, Qian-Ming 1 ; Wang, Hong-Lin 1 ; Yan, Shuang 1 ; Xie, Pu 1 ; Qi, Yong 1 ;
作者机构： 1.Guizhou Acad Agr Sci, Gui Zhou Inst Pomol Sci, Guiyang 550006, Guizhou, Peoples R China
2.Guizhou Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Crop Genet Resources & Germplasm Innovat K, Guiyang 550006, Guizhou, Peoples R China
关键词： Red pitaya; Fruit; Soluble sugars; Vacuole; Overexpression
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）
ISSN： 0981-9428
年卷期： 2025 年 228 卷
页码：
收录情况： SCI
摘要： Soluble sugars are considered to be the most important components to determine the flavor and quality of fleshy fruits. Tonoplast sugar transports (TSTs) mediate sugar uptake into vacuoles and play pivotal roles in plant growth and development. Herein, we performed expression analysis of red pitaya TSTs, selected a candidate isoform HpTST1 and verified its transport activities and biological functions. During red pitaya fruit development, we observed a significant rise in glucose and fructose levels, which were the primary soluble sugars in ripe fruit. Among four HpTSTs, HpTST1 presented the highest expression of an isoform and showed the up-regulation expression pattern that correlated soluble sugars accumulation. Histochemical analysis using a promoter-driven GUS reporter in Arabidopsis indicated that HpTST1 was widely expressed across most source and sink tissues. Subcellular localization studies confirmed that HpTST1 was targeted to the tonoplast in plant cells. Functional assays in baker's yeast demonstrated that HpTST1 was capable of transporting monosaccharides (glucose, fructose and 2-deoxy-D-glucose), sucrose and its analogue esculin. Transient expression of HpTST1 in strawberry fruits significantly increased sucrose, glucose and fructose levels. Moreover, heterologous expression of HpTST1 in tomatoes resulted in an enhanced plant growth and accelerated flowering process. Importantly, compared to wild-type, transgenic lines showed heavier fruit weight by 23.8 %, higher levels of soluble solid contents by 22.7 %, fructose and glucose contents by 35.6-44.4 %. Our research suggests that HpTST1 is a positive regulator of soluble sugars accumulation in red pitaya fruits, thus enhancing our understanding of molecular mechanisms regulating soluble sugars accumulation in fruit vacuoles.
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