miR-34a targets the inhibin beta B gene, promoting granulosa cell apoptosis in the porcine ovary
文献类型： 外文期刊
作者： Tu, F. 1 ; Pan, Z. X. 1 ; Yao, Y. 1 ; Liu, H. L. 1 ; Liu, S. R. 2 ; Xie, Z. 1 ; Li, Q. F. 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Jiangsu, Peoples R China
2.Xinjiang Acad Agr & Reclamat Sci, Anim Husb & Vet Inst, Shihezi, Peoples R China
关键词： miR-34a;INHBB;Apoptosis;Granulosa cells
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2014 年 13 卷 2 期
页码：
收录情况： SCI
摘要： During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in pig ovarian follicles. In addition, inhibin beta B was found to be a miR-34a target gene, based on luciferase reporter assays, quantitative RTPCR and Western blotting. Taken together, our data indicate that miR-34a plays an important role in granulosa cell apoptosis by targeting the INHBB gene in the porcine ovary.
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