文献类型： 外文期刊

作者： Li, F. ¹ ; Chen, X. ² ; Pi, W. ² ; Liu, C. ² ; Shi, Z. ³ ;

作者机构： 1.Shanghai Jiao Tong Univ, Xinhua Hosp, Dev Biol Res Ctr, Sch Med, Shanghai 200025, Peoples R China

2.Shanghai Jiao Tong Univ, Xinhua Hosp, Dev Biol Res Ctr, Sch Med, Shanghai 200025, Peoples R China; Xinjiang Acad Agr & Reclamat Sci, Anim Husb & Vet Inst, Shihezi, Xinjiang, Peoples R China; S China Agr Univ, Dept Anim Sci, Guangzhou, Guangdong, Peoples R China

3.Shanghai Jiao Tong Univ, Xinhua Hosp, Dev Biol Res Ctr, Sch Med, Shanghai 200025, Peoples R China; Xinjiang Acad Agr & Reclamat Sci, Ani

期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）

ISSN： 0936-6768

年卷期： 2007 年 42 卷 6 期

页码：

收录情况： SCI

摘要： The objectives of this study were to use ultrasound-guided transvaginal follicular aspiration to develop a technique for preservation of Nanyang Yellow cattle germ plasm. In Nanyang Yellow heifers, aspiration twice per week yielded a higher mean numbers of aspirated follicles, recovered oocytes and viable oocytes per session than once-weekly aspiration. Intriguingly, twice-weekly aspiration from Holstein heifers yielded a higher mean numbers of aspirated follicles, recovered oocytes and viable oocytes per session than in Nanyang Yellow heifers. Moreover, the oocyte recovery rate was significantly higher in Holstein heifers than in Nanyang Yellow heifers. Importantly, similar rates of embryos undergoing cleavage division were observed in Nanyang Yellow and Holstein, as was the number of viable day 8 blastocysts. These observations demonstrate that twice-weekly aspiration harvested the similar proportions of viable oocytes from Nanyang Yellow and Holstein follicles, and that twice-weekly aspiration yields a higher number of viable oocytes than once-weekly aspiration in Nanyang Yellow heifers. The results also suggest that the combination of ultrasound-guided transvaginal follicular aspiration and in vitro production is an effective and practical strategy for establishment of a germ plasm preservation programme for Nanyang Yellow cattle.