文献类型： 外文期刊

作者： Das, Palashpriya ¹ ; Yang, Xin-Ping ² ; Ma, Luyan Z. ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Biofilm Res Grp, Beijing 100101, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi, Peoples R China

关键词： biosurfactant;rhamnolipid congeners;emulsification;thin layer chromatography;MRL-DRL proportion;antimicrobial action;biofilm disruption

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2014 年 5 卷

页码：

收录情况： SCI

摘要： Rhamnolipid biosurfactants produced mainly by Pseudomonas sp. had been reported to possess a wide range of potential industrial application. These biosurfactants are produced as monorhamnolipid (MRL) and di-rhamnolipid (DRL) congeners. The present study deals with rhamnolipid biosurfactants produced by three bacterial isolates from crude oil. Biosurfactants produced by one of the strains (named as IMP67) was found to be very efficacious based on its critical micelle concentration value and hydrocarbon emulsification property. Strikingly, antimicrobial, and anti-biofilm potential of this biosurfactant were higher than biosurfactants produced by other two strains. Thin layer chromatography analysis and rhamnose quantification showed that the rhamnolipids of IMP67 had more MRL congeners than biosurfactants of the other two strains. Emulsification and antimicrobial actions were affected by manual change of MRL and DRL congener proportions. Increase of MRL proportion enhanced emulsification index and antimicrobial property to Gram negative bacteria. This result indicated that the ratio of MRL and DRL affected the emulsification potentials of rhamnolipids, and suggested that high emulsification potentials might enhance rhamnolipids to penetrate the cell wall of Gram negative bacteria. In line with this finding, rhamnolipids of IMP67 also reduced the MIC of some antibiotics against bacteria, suggesting their synergistic role with the antibiotics.