文献类型： 外文期刊

作者： Peng, Sa ¹ ; Feng, Na ¹ ; Guo, Meili ¹ ; Chen, Yuehua ² ; Guo, Qinghua ¹ ;

作者机构： 1.Second Mil Med Univ, Dept Pharmacognosy, Coll Pharm, Shanghai 200433, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Econ Crop, Urumqi 830000, Peoples R China

关键词： genetic variation;Carthamus tinctorius;Carthamus lanatus;SRAP

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

ISSN： 0305-1978

年卷期： 2008 年 36 卷 7 期

页码：

收录情况： SCI

摘要： Genetic diversity of 23 populations of Carthamus tinctorius L and two populations of Carthamus lanatus L. in China was investigated using Sequence-related Amplified Polymorphism (SRAP). All populations could be uniquely distinguished by 30 primer combinations with 483 bands and 274 polymorphic bands which generated 57% of polymorphic ratio. Unweighed pair-group method of with arithmetical averages (UPGMA) cluster analysis enabled construction of a dendrogram for estimating genetic distances among different populations. The extreme variation was observed when No. 4 cultivated and No. 13 wild population of C. lanatus were grouped at GS = 0.58, and separated from 23 populations of C. tinctorius at GS = 0.10. The result suggested that the cultivated and wild populations of C. lanatus had close relationship with each other and far relationship with C. tinctorius. Dendrogram also revealed a large genetic variation in 23 C. tinctorius populations; different primer combinations allowed them distinctly distinguished one from others with relatively low genetic similarity. Furthermore, five typical representative fragments in C. lanatus were obtained by four most informative primer combinations, which provided a possibility to distinguish C. lanatus from the C. tinctorius evidently. (C) 2008 Elsevier Ltd. All rights reserved.