文献类型： 外文期刊
作者： AI, XL 1 ; CHEN, XG 1 ; JI, Q 1 ; LONG, T 1 ; YU, H 1 ; XI, FG 1 ; DONG, P 1 ; SHI, YH 1 ;
作者机构： 1.XINJIANG ACAD AGR SCI,INST MICROBIOL,URUMQI 830000,PEOPLES R CHINA
关键词： CDNA SYNTHESIS; PCR AMPLIFICATION; GENE EXPRESSION OF BFSH
期刊名称：SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES （ 影响因子：0.479； ）
ISSN： 1001-652X
年卷期： 1995 年 38 卷 11 期
页码：
收录情况： SCI
摘要： A cDNA library was prepared from mRNA extracted from bovine pituitaries. beta-subunit DNA of bFSH was amplified from cDNA using Polymerase Chain Reaction (PCR). The sequence of amino acid encoded by cDNA was the same as that of natural bFSH by sequencing. Signal peptide DNA fragment of bFSH was deleted by PCR mediated mutagenesis, to which PGEX-2T was ligated, yielding a high expression fusion protein of 40kD. A recombinant bFSH fusion protein was extracted using thrombin, yielding a pure bFSH of 14kD, showing positive reaction using Western blot analysis, and some activation by biological tests with the mouse and the rabbit.
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