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The interaction of soybean reticulon homology domain protein (GmRHP) with Soybean mosaic virus encoded P3 contributes to the viral infection

文献类型: 外文期刊

作者: Cui, Xiaoyan 1 ; Lu, Lu 1 ; Wang, Ying 1 ; Yuan, Xingxing 1 ; Chen, Xin 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Inst Ind Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China

2.Yangzhou Univ, Sch Hort & Plant Protect, Dept Hort, 48 East Wenhui Rd, Yangzhou 225009, Jiangsu, Peoples R China

关键词: Interaction;Soybean mosaic virus;P3;GmRHP;Infection

期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )

ISSN: 0006-291X

年卷期: 2018 年 495 卷 3 期

页码:

收录情况: SCI

摘要: Soybean mosaic virus.(SMV), a member of the Potyvirus genus, is a prevalent and devastating viral pathogen in soybean-growing regions worldwide. Potyvirus replication occurs in the 6K2-induced viral replication complex at endoplasmic reticulum exit sites. Potyvirus-encoded P3 is also associated with the endoplasmic reticulum and is as an essential component of the viral replication complex, playing a key role in viral replication. This study provides evidence that the soybean (Glycine max) reticulon homology domain protein (designated as GmRHP) interacts with SMV-P3 by using a two-hybrid yeast system to screen a soybean cDNA library. A bimolecular fluorescence complementation assay further confirmed the interaction, which occurred on the cytomembrane, endoplasmic reticulum and cytoskeleton in Nicotiana benthamiana cells. The transient expression of GmRHP can promote the coupling of Turnip mosaic virus replication and cell-to-cell movement in N. benthamiana. The interaction between the membrane protein SMV-P3 and GmRHP may contribute to the potyvirus infection, and GmRHP may be an essential host factor for P3's involvement in potyvirus replication. (C) 2017 Elsevier Inc. All rights reserved.

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