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Detoxification of diphenyl ether herbicide lactofen by Bacillus sp Za and enantioselective characteristics of an esterase gene lacE

文献类型： 外文期刊

作者： Zhang, Jing 1 ; Lu, Luyao 1 ; Chen, Feng 1 ; Chen, Lingling 1 ; Yin, Jingang 3 ; Huang, Xing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Minist Agr, Key Lab Agr Environm Microbiol, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjing 210014, Jiangsu, Peoples R China

3.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Synthet Biotechnol, Shanghai 200237, Peoples R China

关键词： Lactofen;Bacillus sp za;Detoxification;Esterase;Enantioselectivity

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：10.588； 五年影响因子：10.129 ）

ISSN： 0304-3894

年卷期： 2018 年 341 卷

页码：

收录情况： SCI

摘要： A bacterial strain Za capable of degrading diphenyl ether herbicide lactofen was isolated and identified as Bacillus sp. This strain could degrade 94.8% of 50 mg L-1 lactofen after 4 days of inoculation in flasks. It was revealed that lactofen was initially hydrolyzed to desethyl lactofen, which was further transformed to acifluorfen, followed by the reduction of the nitro group to yield aminoacifluorfen. The phytotoxicity of the transformed product aminoacifluorfen to maize was decreased significantly compared with the lactofen. A gene lacE, encoding an esterase responsible for lactofen hydrolysis to desethyl lactofen and acifluorfen continuously, was cloned from Bacillus sp. Za. The deduced amino acid belonging to the esterase family VII contained a typical Ser-His-Asp/Glu catalytic triad and the conserved motifs GXSXG. The purified recombinant protein LacE displayed maximal esterase activity at 40 degrees C and pH 7.0. Additionally, LacE had broad substrate specificity and was capable of hydrolyzing p-nitrophenyl esters. The enantioselectivity of LacE during lactofen degradation was further studied, and the results indicated that the (S)-(+)-lactofen was degraded faster than the (R)-(-)-lactofen, which could illustrate the reported phenomenon that (S)-(+)-lactofen was preferentially degraded in soil and sediment. (C) 2017 Elsevier B.V. All rights reserved.

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