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RNA-seq analysis reveals a key role of brassinolide-regulated pathways in NaCl-stressed cotton

文献类型: 外文期刊

作者: Shu, H. M. 1 ; Guo, S. Q. 1 ; Gong, Y. Y. 1 ; Jiang, L. 1 ; Zhu, J. W. 1 ; Ni, W. C. 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Inst Ind Crops, Key Lab Cotton & Rapeseed, Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

关键词: chlorophyll;differentially expressed genes;gene ontology enrichment analysis;photosynthesis;proteins

期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )

ISSN: 0006-3134

年卷期: 2017 年 61 卷 4 期

页码:

收录情况: SCI

摘要: Brassinolide (BL) alleviates salt injury in cotton seedlings; however, little is known about the molecular mechanisms of this response. In this study, digital gene expression analysis was performed to better understand the regulatory pathways of BL in NaCl-stressed cotton (Gossypium hirsutum L.). Compared with control plants (CK), a total of 1 162 and 7 659 differentially expressed genes (DEGs) were detected in the leaves and roots of NaCl-treated plants, respectively. Most of the DEGs in NaCl-treated plants, compared to CK, were regulated by BL. Moreover, expression patterns of DEGs in BL+NaCl treated plants were similar to those in CK plants; however, the responses of DEGs in the leaves and roots of NaCl-treated plants to BL differed. In the roots, BL-regulated DEGs were involved in protein biosynthesis, whereas in the leaves, BL promoted photosynthesis in NaCl-stressed cotton. BL treatment also significantly increased the overall biomass, chlorophyll a + b content in leaves, and the protein content in roots in NaCl-stressed cotton. The downregulation of stress-responsive genes in BL+NaCl-stressed leaves was also found. These results suggest that BL can alleviate NaCl injury in cotton plants.

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