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Two distinctive beta subunits are separately involved in two binding sites of imidacloprid with different affinities in Locusta migratoria manilensis

文献类型： 外文期刊

作者： Bao, Haibo 1 ; Liu, Yang 1 ; Zhang, Yixi 1 ; Liu, Zewen 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, 50 St Zhongling, Nanjing 210014, Jiangsu, Peoples R China

关键词： Locusta migratoria;Nicotinic acetylcholine receptors;beta subunit;Imidacloprid binding

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2017 年 140 卷

页码：

收录情况： SCI

摘要： Due to great diversity of nicotinic acetylcholine receptor (nAChR) subtypes in insects, one 13 subunit may be contained in numerous nAChR subtypes. In the locust Locusta migratoria, a model insect species with agricultural importance, the third beta subunits (Loc beta 3) was identified in this study, which reveals at least three beta subunits in this insect species. Imidacloprid was found to bind nAChRs in L. migratoria central nervous system at two sites with different affinities, with K-d values of 0.16 and 10.31 nM. The specific andsera (L1-1, L2-1 and L3-1) were raised against fusion proteins at the large cytoplasmic loop of Loc beta, Loc beta 2 and Loc beta 3 respectively. Specific immunodepletion of Loc beta 1 with antiserum L1-1 resulted in the selective loss of the low affinity binding site for imidacloprid, whereas the immunodepletion of Lo beta 3 with L3-1 caused the selective loss of the high affinity site. Dual immunodepletion with L1-1 and L3-1 could completely abolish imidacloprid binding. In contrast, the immunodepletion of Loc beta 2 had no significant effect on the specific [H-3]imidacloprid binding. Taken together, these data indicated that Loc beta 1 and Loc beta 3 were respectively contained in the low-and high-affinity binding sites for imidacloprid in L. migratoria, which is different to the previous finding in Nilaparvata lugens that NI beta 1 was in two binding sites for imidacloprid. The involvement of two beta subunits separately in two binding sites may decrease the risk of imidacloprid resistance due to putative point mutations in beta subunits in L migratoria. (C) 2017 Elsevier Inc. All rights reserved.

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[1]Selective actions of Lynx proteins on different nicotinic acetylcholine receptors in the locust, Locusta migratoria manilensis. Wang, Xin,Bao, Haibo,Sun, Huahua,Zhang, Yixi,Liu, Qinghong,Liu, Zewen,Bao, Haibo,Fang, Jichao.

[2]IPPA08 allosterically enhances the action of imidacloprid on nicotinic acetylcholine receptors. Bao, Haibo,Fang, Jichao,Bao, Haibo,Zhang, Yixi,Wang, Yunchao,Liu, Zewen,Shao, Xusheng,Zhang, Yixi,Cheng, Jiagao,Xu, Xiaoyong,Li, Zhong.

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